Human IGFBP-4 ELISA Kit

EK1277

Human IGFBP-4 ELISA Kit

$245.00 – $350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity6.51 pg/mL
Array Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery81% – 115%
Average Recovery94%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity6.51 pg/mL
Array Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery81% – 115%
Average Recovery94%
Intra Precision2.9% – 4.0%
Inter Precision2.8% – 4.3%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody
Human TRAIL/TNFSF10 freeze-dried standard
TRAIL/TNFSF10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGFBP4

IGFBP4 Target Infomation Overview

  • Target Symbol: IGFBP4, insulin like growth factor binding protein 4
  • Gene Groups: Insulin like growth factor binding proteins
  • Alias: IBP4; BP-4; HT29-IGFBP; IGFBP-4
  • Alias Names: IGF-binding protein 4; insulin-like growth factor-binding protein 4; insulin-like growth factor binding protein 4

IGFBP4, insulin like growth factor binding protein 4 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IGFBP4 Target Information

  • Target Symbol: IGFBP4, insulin like growth factor binding protein 4
  • Alias:
    • BP-4
    • HT29-IGFBP
    • IBP-4
    • IBP4
    • IGF-binding protein 4
    • IGFBP-4
    • insulin-like growth factor binding protein 4
    • insulin-like growth factor-binding protein 4
  • NCBI_Gene: 3487
  • UniProtKB: P22692

Human IGFBP4 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Predicted to act upstream of or within several processes, including positive regulation of signal transduction; regulation of glucose metabolic process; and type B pancreatic cell proliferation. Predicted to be located in endoplasmic reticulum lumen and extracellular region. Predicted to be active in extracellular space.

Mouse Igfbp4 Target Information

Mouse Igfbp4 Predicted Functions

Enables insulin-like growth factor binding activity. Acts upstream of or within several processes, including positive regulation of signal transduction; regulation of glucose metabolic process; and type B pancreatic cell proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; early embryo; female reproductive system; limb; and lung. Orthologous to human IGFBP4 (insulin like growth factor binding protein 4).

Rat Igfbp4 Target Information

  • Target Symbol: Igfbp4, insulin-like growth factor binding protein 4
  • Alias:
    • IBP-4
    • IBP4
    • IGF-binding protein 4
    • IGF-BP4
    • IGFBP-4
    • insulin-like growth factor-binding protein 4
  • NCBI_Gene: 360622
  • UniProtKB: P21744

Rat Igfbp4 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in inflammatory response. Predicted to be active in extracellular space. Orthologous to human IGFBP4 (insulin like growth factor binding protein 4).

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Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

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