Human IGFBP-6 ELISA Kit

$245.00 – $350.00

Related Target	IGFBP6
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	8.38 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 103%
Average Recovery	96%

ELISA Kit Detail Information

Related Target	IGFBP6
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	8.38 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 103%
Average Recovery	96%
Intra Precision	0.8% – 1.2%
Inter Precision	0.8% – 2.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-6 monoclonal antibody Human IGFBP-6 freeze-dried standard IGFBP-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGFBP6

IGFBP6 Target Infomation Overview

Target Symbol: IGFBP6, insulin like growth factor binding protein 6
Gene Groups: Insulin like growth factor binding proteins
Alias Names: insulin-like growth factor binding protein 6

IGFBP6, insulin like growth factor binding protein 6 Target Infomation by Species

Human
Mouse
Rat

Human IGFBP6 Target Information

Target Symbol: IGFBP6, insulin like growth factor binding protein 6
Alias:
- IBP-6
- IBP6
- IGF binding protein 6
- IGF-binding protein 6
- IGFBP-6
- insulin-like growth factor binding protein 6
- insulin-like growth factor-binding protein 6
NCBI_Gene: 3489
UniProtKB: P24592

Human IGFBP6 Predicted Functions

Enables identical protein binding activity and insulin-like growth factor II binding activity. Involved in cell migration and positive regulation of stress-activated MAPK cascade. Part of insulin-like growth factor binary complex. Implicated in obesity. Biomarker of breast cancer; in situ carcinoma; leiomyoma; and neovascular inflammatory vitreoretinopathy.

Mouse Igfbp6 Target Information

Target Symbol: Igfbp6, insulin-like growth factor binding protein 6
Alias:
- IGFBP-6
NCBI_Gene: 16012
- UniprotKB: P47880

Mouse Igfbp6 Predicted Functions

Predicted to enable fibronectin binding activity; identical protein binding activity; and insulin-like growth factor binding activity. Predicted to be involved in cell migration; positive regulation of stress-activated MAPK cascade; and regulation of insulin-like growth factor receptor signaling pathway. Located in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including blastocyst; compacted morula; genitourinary system; liver; and spleen. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP6 (insulin like growth factor binding protein 6).

Rat Igfbp6 Target Information

Target Symbol: Igfbp6, insulin-like growth factor binding protein 6
Alias:
- IBP-6
- IGF-binding protein 6
- IGF-BP6
- IGFBP-6
- insulin-like growth factor-binding protein 6
- RBP6A
- RBP6G
NCBI_Gene: 25641
UniProtKB: P35572

Rat Igfbp6 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in cell migration; positive regulation of stress-activated MAPK cascade; and regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus and extracellular space. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP6 (insulin like growth factor binding protein 6).

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