EK1287

Human IGFBP-7 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity4.67 pg/mL
Array Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery85% – 112%
Average Recovery104%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity4.67 pg/mL
Array Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery85% – 112%
Average Recovery104%
Intra Precision2.0% – 3.2%
Inter Precision2.4% - 3.2%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-7 monoclonal antibody
Human IGFBP-7 freeze-dried standard
IGFBP-7 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(22×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IGFBP-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGFBP7

IGFBP7 Target Infomation Overview

  • Target Symbol: IGFBP7, insulin like growth factor binding protein 7
  • Gene Groups: I-set domain containing
  • Alias: MAC25; IGFBP-7; PSF; FSTL2
  • Alias Names: insulin-like growth factor binding protein 7

IGFBP7, insulin like growth factor binding protein 7 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IGFBP7 Target Information

  • Target Symbol: IGFBP7, insulin like growth factor binding protein 7
  • Alias:
    • AGM
    • angiomodulin
    • FSTL2
    • IBP-7
    • IGF-binding protein 7
    • IGFBP-7
    • IGFBP-7v
    • IGFBP-rP1
    • IGFBPRP1
    • insulin-like growth factor binding protein 7
    • insulin-like growth factor-binding protein 7
    • MAC25
    • PGI2-stimulating factor
    • prostacyclin-stimulating factor
    • PSF
    • RAMSVPS
    • TAF
    • tumor-derived adhesion factor
  • NCBI_Gene: 3490
  • UniProtKB: Q16270

Human IGFBP7 Predicted Functions

Predicted to enable insulin-like growth factor binding activity. Predicted to be an extracellular matrix structural constituent. Involved in cell adhesion. Located in extracellular exosome. Colocalizes with collagen-containing extracellular matrix.

Mouse Igfbp7 Target Information

Mouse Igfbp7 Predicted Functions

Predicted to enable insulin-like growth factor binding activity. Predicted to be an extracellular matrix structural constituent. Acts upstream of or within inner ear development. Located in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; brain; cardiovascular system; eye; and genitourinary system. Orthologous to human IGFBP7 (insulin like growth factor binding protein 7).

Rat Igfbp7 Target Information

Rat Igfbp7 Predicted Functions

Predicted to enable insulin-like growth factor binding activity. Involved in several processes, including embryo implantation; response to cortisol; and response to retinoic acid. Located in extracellular space. Biomarker of renal cell carcinoma. Orthologous to human IGFBP7 (insulin like growth factor binding protein 7).

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