Human IGFBP-1 ELISA Kit

EK1255A

Human IGFBP-1 ELISA Kit

$319.00 – $449.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample VolumeNormal serum and plasma samples require a 100-fold dilution,cell culture supernatant: 100 μL
Sensitivity0.59 pg/mL
Array Range9.38 pg/mL - 600 pg/mL
Assay Time3.5 h
Recovery82% - 113%
Average Recovery99%
SKU: EK1255A Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample VolumeNormal serum and plasma samples require a 100-fold dilution,cell culture supernatant: 100 μL
Sensitivity0.59 pg/mL
Array Range9.38 pg/mL - 600 pg/mL
Assay Time3.5 h
Recovery82% - 113%
Average Recovery99%
Intra Precision2.68% - 4.13%
Inter Precision1.41% - 3.21%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Assay Buffer ( 1×)
Substrate TMB
Detect Antibody
Streptavidin-HRP
Control (High/Low)
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IGFBP- 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IGFBP- 1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IGFBP- 1 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IGFBP- 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Related Targets

IGFBP1

IGFBP1 Target Infomation Overview

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Gene Groups: Insulin like growth factor binding proteins
  • Alias: IGF-BP25; AFBP; hIGFBP-1; PP12
  • Previous Names: IBP1
  • Alias Names: placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1

IGFBP1, insulin like growth factor binding protein 1 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IGFBP1 Target Information

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Alias:
    • AFBP
    • alpha-pregnancy-associated endometrial globulin
    • amniotic fluid binding protein
    • binding protein-25
    • binding protein-26
    • binding protein-28
    • growth hormone independent-binding protein
    • hIGFBP-1
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBP-1
    • insulin-like growth factor binding protein 1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
    • PP12
  • NCBI_Gene: 3484
  • UniProtKB: P08833

Human IGFBP1 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.

Mouse Igfbp1 Target Information

Mouse Igfbp1 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including blastocyst; early embryo; liver; and secondary oocyte. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

Rat Igfbp1 Target Information

  • Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
  • Alias:
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBA
    • IGFBP-1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
  • NCBI_Gene: 25685
  • UniProtKB: P21743

Rat Igfbp1 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including insulin receptor signaling pathway; positive regulation of cell growth; and tissue regeneration. Located in extracellular space. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

Products with the Same Target - IGFBP1

Human IGFBP-1 ELISA Kit

$299.00 – $419.00
Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

Reviews

There are no reviews yet.

Be the first to review “Human IGFBP-1 ELISA Kit”

Your email address will not be published. Required fields are marked *

Related products