Human IGFBP-1 ELISA Kit

$245.00 – $350.00

Related Target	IGFBP1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.93 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 118%
Average Recovery	101%

ELISA Kit Detail Information

Related Target	IGFBP1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.93 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 118%
Average Recovery	101%
Intra Precision	4.8% – 5.2%
Inter Precision	3.0% – 5.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-1 monoclonal antibody Human IGFBP-1 freeze-dried standard IGFBP-1 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGFBP-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGFBP1

IGFBP1 Target Infomation Overview

Target Symbol: IGFBP1, insulin like growth factor binding protein 1
Gene Groups: Insulin like growth factor binding proteins
Alias: IGF-BP25; AFBP; hIGFBP-1; PP12
Previous Names: IBP1
Alias Names: placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1

IGFBP1, insulin like growth factor binding protein 1 Target Infomation by Species

Human
Mouse
Rat

Human IGFBP1 Target Information

Target Symbol: IGFBP1, insulin like growth factor binding protein 1
Alias:
- AFBP
- alpha-pregnancy-associated endometrial globulin
- amniotic fluid binding protein
- binding protein-25
- binding protein-26
- binding protein-28
- growth hormone independent-binding protein
- hIGFBP-1
- IBP-1
- IBP1
- IGF-binding protein 1
- IGF-BP25
- IGFBP-1
- insulin-like growth factor binding protein 1
- insulin-like growth factor-binding protein 1
- placental protein 12
- PP12
NCBI_Gene: 3484
UniProtKB: P08833

Human IGFBP1 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.

Mouse Igfbp1 Target Information

Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
Alias:
- IGFBP-1
NCBI_Gene: 16006
- UniprotKB: P47876

Mouse Igfbp1 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including blastocyst; early embryo; liver; and secondary oocyte. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

Rat Igfbp1 Target Information

Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
Alias:
- IBP-1
- IBP1
- IGF-binding protein 1
- IGF-BP25
- IGFBA
- IGFBP-1
- insulin-like growth factor-binding protein 1
- placental protein 12
NCBI_Gene: 25685
UniProtKB: P21743

Rat Igfbp1 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including insulin receptor signaling pathway; positive regulation of cell growth; and tissue regeneration. Located in extracellular space. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

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