Human IL-11 ELISA Kit

$245.00 – $350.00

Related Target	IL11
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	5.01 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	84% – 118%
Average Recovery	99%

ELISA Kit Detail Information

Related Target	IL11
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	5.01 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	84% – 118%
Average Recovery	99%
Intra Precision	5.6% – 8.3%
Inter Precision	4.4% – 7.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-11 monoclonal antibody Human IL-11 freeze-dried standard IL-11 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-11 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-11 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-11 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL11

IL11 Target Infomation Overview

Target Symbol: IL11, interleukin 11
Gene Groups: Interleukins; Interleukin 6 type cytokine family
Alias: IL-11; AGIF
Alias Names: adipogenesis inhibitory factor; oprelvekin

IL11, interleukin 11 Target Infomation by Species

Human
Mouse
Rat

Human IL11 Target Information

Target Symbol: IL11, interleukin 11
Alias:
- adipogenesis inhibitory factor
- AGIF
- IL-11
- interleukin-11
- oprelvekin
NCBI_Gene: 3589
UniProtKB: P20809

Human IL11 Predicted Functions

Enables growth factor activity. Involved in positive regulation of cell population proliferation. Acts upstream of or within several processes, including negative regulation of hormone secretion; positive regulation of MAPK cascade; and positive regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in cytoplasm.

Mouse Il11 Target Information

Mouse Il11 Predicted Functions

Enables cytokine activity and interleukin-11 receptor binding activity. Acts upstream of or within positive regulation of cell population proliferation. Located in cytoplasm. Orthologous to human IL11 (interleukin 11).

Rat Il11 Target Information

Target Symbol: Il11, interleukin 11
Alias:
- IL-11
- interleukin-11
NCBI_Gene: 171040
- UniprotKB: G3V890
- UniprotKB: Q99MF5

Rat Il11 Predicted Functions

Predicted to enable cytokine activity; growth factor activity; and interleukin-11 receptor binding activity. Predicted to be involved in positive regulation of MAPK cascade and positive regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of hormone secretion; positive regulation of protein phosphorylation; and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Predicted to be active in cytoplasm. Orthologous to human IL11 (interleukin 11).

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