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Human IL-12p70 High Sensitivity ELISA Kit
$380.00 – $480.00
| SPECIES | Human |
|---|---|
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.04 pg/mL |
| Range | 0.39 pg/mL – 25 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 104% |
| Average Recovery | 0.97 |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.04 pg/mL |
| Range | 0.39 pg/mL – 25 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 104% |
| Average Recovery | 0.97 |
| Intra Precision | 4.7% – 4.9% |
| Inter-Precision | 4.2% – 6.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-12p70 monoclonal antibody Human IL-12p70 high sensitivity freeze-dried standard IL-12p70 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-12p70 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-12p70 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-12p70 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
