Human IL-17F ELISA Kit

$350.00$450.00

Download:
TARGETSIL17F
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.21 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery78% – 114%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.21 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery78% – 114%
Average Recovery0.93
Intra Precision4.4% – 5.3%
Inter-Precision2.5%-8.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17F monoclonal antibody
Human IL-17F freeze-dried standard
IL-17F detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17F antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17F present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17F in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL17F

IL17F Target Infomation Overview

  • Target Symbol: IL17F, interleukin 17F
  • Gene Groups: Interleukins
  • Alias: IL-17F; ML-1; ML1

IL17F, interleukin 17F Target Infomation by Species

HumanMouseRat

Human IL17F Target Information

Human IL17F Predicted Functions

Enables cytokine activity; cytokine binding activity; and protein homodimerization activity. Involved in several processes, including interleukin-17-mediated signaling pathway; negative regulation of angiogenesis; and regulation of cytokine production. Predicted to be located in extracellular region. Implicated in chronic mucocutaneous candidiasis.

Mouse Il17f Target Information

Mouse Il17f Predicted Functions

Enables cytokine receptor binding activity; protein heterodimerization activity; and protein homodimerization activity. Involved in defense response to bacterium and positive regulation of gene expression. Acts upstream of or within positive regulation of cytokine production involved in inflammatory response and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Human ortholog(s) of this gene implicated in chronic mucocutaneous candidiasis. Orthologous to human IL17F (interleukin 17F).

Rat Il17f Target Information

Rat Il17f Predicted Functions

Predicted to enable cytokine binding activity; protein dimerization activity; and signaling receptor binding activity. Predicted to be involved in several processes, including defense response to bacterium; interleukin-17-mediated signaling pathway; and regulation of cytokine production. Predicted to act upstream of or within positive regulation of cytokine production involved in inflammatory response and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Human ortholog(s) of this gene implicated in chronic mucocutaneous candidiasis. Orthologous to human IL17F (interleukin 17F).

IL17F Target News

    3.7
    A role of NR4A2 in Graves’ disease: regulation of Th17/Treg
    ENDOCRINE
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target

    EK2196

    $350.00$450.00