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Human IL-17F ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.21 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 78% – 114% |
| Average Recovery | 0.93 |
| Intra Precision | 4.4% – 5.3% |
| Inter-Precision | 2.5%-8.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17F monoclonal antibody Human IL-17F freeze-dried standard IL-17F detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17F antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17F present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17F in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL17F
IL17F Target Infomation Overview
- Target Symbol: IL17F, interleukin 17F
- Gene Groups: Interleukins
- Alias: IL-17F; ML-1; ML1
IL17F, interleukin 17F Target Infomation by Species
HumanMouseRat
Human IL17F Target Information
- Target Symbol: IL17F, interleukin 17F
- Alias:
- CANDF6
- cytokine ML-1
- IL-17F
- IL-24
- interleukin-17F
- interleukin-24
- ML-1
- ML1
- mutant IL-17F
- NCBI_Gene: 112744
- UniProtKB: Q96PD4
Human IL17F Predicted Functions
Enables cytokine activity; cytokine binding activity; and protein homodimerization activity. Involved in several processes, including interleukin-17-mediated signaling pathway; negative regulation of angiogenesis; and regulation of cytokine production. Predicted to be located in extracellular region. Implicated in chronic mucocutaneous candidiasis.
Mouse Il17f Target Information
- Target Symbol: Il17f, interleukin 17F
- Alias:
- C87042
- expressed sequence C87042
- NCBI_Gene: 257630
Mouse Il17f Predicted Functions
Enables cytokine receptor binding activity; protein heterodimerization activity; and protein homodimerization activity. Involved in defense response to bacterium and positive regulation of gene expression. Acts upstream of or within positive regulation of cytokine production involved in inflammatory response and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Human ortholog(s) of this gene implicated in chronic mucocutaneous candidiasis. Orthologous to human IL17F (interleukin 17F).
Rat Il17f Target Information
- Target Symbol: Il17f, interleukin 17F
- Alias:
- IL-17F
- interleukin-17F
- LOC301291
- MGC114402
- NCBI_Gene: 301291
Rat Il17f Predicted Functions
Predicted to enable cytokine binding activity; protein dimerization activity; and signaling receptor binding activity. Predicted to be involved in several processes, including defense response to bacterium; interleukin-17-mediated signaling pathway; and regulation of cytokine production. Predicted to act upstream of or within positive regulation of cytokine production involved in inflammatory response and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Human ortholog(s) of this gene implicated in chronic mucocutaneous candidiasis. Orthologous to human IL17F (interleukin 17F).
