EK1132

Human IL-1ra/IL-1F3 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity7.71 pg/mL
Array Range39.06 pg/mL – 2500 pg/mL
Assay Time3.5 h
Recovery87% – 108%
Average Recovery101%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity7.71 pg/mL
Array Range39.06 pg/mL – 2500 pg/mL
Assay Time3.5 h
Recovery87% – 108%
Average Recovery101%
Intra Precision2.5% – 3.8%
Inter Precision2.2% – 7.3%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1ra/IL-1F3A monoclonal antibody
Human IL-1ra/IL-1F3A freeze-dried standard
IL-1ra/IL-1F3A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-1ra antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1ra present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1ra in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL1RN

IL1RN Target Infomation Overview

  • Target Symbol: IL1RN, interleukin 1 receptor antagonist
  • Gene Groups: Interleukins; Interleukin receptors
  • Alias: IL1RA; ICIL-1RA; IL1F3; IRAP; IL-1RN; MGC10430
  • Alias Names: interleukin-1 receptor antagonist protein; intracellular interleukin-1 receptor antagonist

IL1RN, interleukin 1 receptor antagonist Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL1RN Target Information

  • Target Symbol: IL1RN, interleukin 1 receptor antagonist
  • Alias:
    • DIRA
    • ICIL-1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RN (IL1F3)
    • interleukin-1 receptor antagonist protein
    • intracellular IL-1 receptor antagonist type II
    • intracellular interleukin-1 receptor antagonist (icIL-1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • type II interleukin-1 receptor antagonist
  • NCBI_Gene: 3557
  • UniProtKB: P18510

Human IL1RN Predicted Functions

Enables cytokine receptor binding activity. Involved in several processes, including acute-phase response; negative regulation of heterotypic cell-cell adhesion; and negative regulation of interleukin-1-mediated signaling pathway. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); chronic kidney disease (multiple); glomerulonephritis (multiple); lung disease (multiple); and obesity (multiple).

Mouse Il1rn Target Information

Mouse Il1rn Predicted Functions

Predicted to enable cytokine receptor binding activity. Acts upstream of or within insulin secretion and lipid metabolic process. Located in extracellular region and vesicle. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).

Rat Il1rn Target Information

Rat Il1rn Predicted Functions

Enables interleukin-1 receptor binding activity. Involved in several processes, including cellular response to norepinephrine stimulus; chronic inflammatory response to antigenic stimulus; and fever generation. Located in extracellular space. Used to study adult respiratory distress syndrome; anti-basement membrane glomerulonephritis; hypertension; silicosis; and transient cerebral ischemia. Biomarker of encephalitis and pneumonia. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).

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