Human IL-21 ELISA Kit

$350.00$450.00

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TARGETSIL21
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity11.99 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 112%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity11.99 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 112%
Average Recovery1.02
Intra Precision4.5% – 5.1%
Inter-Precision7.2% – 7.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-21 monoclonal antibody
Human IL-21 freeze-dried standard
IL-21 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-21 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-21 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL21

IL21 Target Infomation Overview

  • Target Symbol: IL21, interleukin 21
  • Gene Groups: Interleukins
  • Alias: Za11; IL-21

IL21, interleukin 21 Target Infomation by Species

HumanMouseRat

Human IL21 Target Information

Human IL21 Predicted Functions

Enables interleukin-2 receptor binding activity. Involved in several processes, including positive regulation of interleukin-17 production; positive regulation of natural killer cell mediated cytotoxicity; and tyrosine phosphorylation of STAT protein. Acts upstream of or within positive regulation of B cell proliferation; positive regulation of T cell proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular region. Implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Biomarker of several diseases, including anogenital venereal wart; autoimmune disease (multiple); dengue hemorrhagic fever; human immunodeficiency virus infectious disease; and tropical spastic paraparesis.

Mouse Il21 Target Information

Mouse Il21 Predicted Functions

Enables cytokine receptor binding activity. Involved in cellular response to virus; lymphocyte activation involved in immune response; and positive regulation of immunoglobulin production. Acts upstream of or within positive regulation of cytokine production; positive regulation of natural killer cell differentiation; and positive regulation of natural killer cell mediated cytotoxicity. Predicted to be located in extracellular space. Is expressed in ductus deferens; epididymis; and testis. Human ortholog(s) of this gene implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Orthologous to human IL21 (interleukin 21).

Rat Il21 Target Information

Rat Il21 Predicted Functions

Predicted to enable interleukin-2 receptor binding activity. Predicted to be involved in several processes, including lymphocyte activation involved in immune response; positive regulation of immune effector process; and tyrosine phosphorylation of STAT protein. Predicted to act upstream of or within positive regulation of cytokine production; positive regulation of lymphocyte activation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Biomarker of periodontal disease. Human ortholog(s) of this gene implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Orthologous to human IL21 (interleukin 21).

IL21 Target News

    5.195
    Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro
    JOURNAL OF ETHNOPHARMACOLOGY
    2.919
    The regulation of Tfh cell differentiation by β-hydroxybutyrylation modification of transcription factor Bcl6
    CHROMOSOMA
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus

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