Human IL-21 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL21
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	11.99 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 112%
Average Recovery	1.02

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	11.99 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 112%
Average Recovery	1.02
Intra Precision	4.5% – 5.1%
Inter-Precision	7.2% – 7.7%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-21 monoclonal antibody Human IL-21 freeze-dried standard IL-21 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×)
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-21 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-21 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL21

IL21 Target Infomation Overview

Target Symbol: IL21, interleukin 21
Gene Groups: Interleukins
Alias: Za11; IL-21

IL21, interleukin 21 Target Infomation by Species

HumanMouseRat

Human IL21 Target Information

Target Symbol: IL21, interleukin 21
Alias:
- CVID11
- IL-21
- interleukin-21
- interleukin-21 isoform
- Za11
NCBI_Gene: 59067
UniProtKB: Q9HBE4

Human IL21 Predicted Functions

Enables interleukin-2 receptor binding activity. Involved in several processes, including positive regulation of interleukin-17 production; positive regulation of natural killer cell mediated cytotoxicity; and tyrosine phosphorylation of STAT protein. Acts upstream of or within positive regulation of B cell proliferation; positive regulation of T cell proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular region. Implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Biomarker of several diseases, including anogenital venereal wart; autoimmune disease (multiple); dengue hemorrhagic fever; human immunodeficiency virus infectious disease; and tropical spastic paraparesis.

Mouse Il21 Target Information

Mouse Il21 Predicted Functions

Enables cytokine receptor binding activity. Involved in cellular response to virus; lymphocyte activation involved in immune response; and positive regulation of immunoglobulin production. Acts upstream of or within positive regulation of cytokine production; positive regulation of natural killer cell differentiation; and positive regulation of natural killer cell mediated cytotoxicity. Predicted to be located in extracellular space. Is expressed in ductus deferens; epididymis; and testis. Human ortholog(s) of this gene implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Orthologous to human IL21 (interleukin 21).

Rat Il21 Target Information

Target Symbol: Il21, interleukin 21
Alias:
- IL-21
- interleukin-21
- LOC365769
NCBI_Gene: 365769
UniProtKB: A3QPB9

Rat Il21 Predicted Functions

Predicted to enable interleukin-2 receptor binding activity. Predicted to be involved in several processes, including lymphocyte activation involved in immune response; positive regulation of immune effector process; and tyrosine phosphorylation of STAT protein. Predicted to act upstream of or within positive regulation of cytokine production; positive regulation of lymphocyte activation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Biomarker of periodontal disease. Human ortholog(s) of this gene implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Orthologous to human IL21 (interleukin 21).