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Human IL-3 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.94 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 87% – 119% |
| Average Recovery | 1.01 |
| Intra Precision | 4.3%-9.5% |
| Inter-Precision | 1.5% – 8.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-3 monoclonal antibody Human IL-3 freeze-dried standard IL-3 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL3
IL3 Target Infomation Overview
- Target Symbol: IL3, interleukin 3
- Gene Groups: Interleukins
- Alias: IL-3; MULTI-CSF; MCGF; MGC79398; MGC79399
- Alias Names: multilineage-colony-stimulating factor; hematopoietic growth factor; P-cell stimulating factor; mast-cell growth factor; colony-stimulating factor, multiple; interleukin 3 (colony-stimulating factor, multiple)
IL3, interleukin 3 Target Infomation by Species
HumanMouseRat
Human IL3 Target Information
- Target Symbol: IL3, interleukin 3
- Alias:
- colony-stimulating factor, multiple
- hematopoietic growth factor
- IL-3
- interleukin-3
- mast cell growth factor
- mast-cell growth factor
- MCGF
- MGC79398
- MGC79399
- MULTI-CSF
- multilineage-colony-stimulating factor
- multipotential colony-stimulating factor
- P-cell stimulating factor
- P-cell-stimulating factor
- NCBI_Gene: 3562
- UniProtKB: P08700
Human IL3 Predicted Functions
Predicted to enable interleukin-3 receptor binding activity. Involved in embryonic hemopoiesis; positive regulation of cell population proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Implicated in Graves’ disease; asthma; and rheumatoid arthritis. Biomarker of several diseases, including Ebola hemorrhagic fever; genital herpes; major depressive disorder; obesity; and psoriasis.
Mouse Il3 Target Information
- Target Symbol: Il3, interleukin 3
- Alias:
- BPA
- colony stimulating factor, multi (contains Il-3)
- Csfmu
- HCGF
- Il-3
- MCGF
- PSF
- NCBI_Gene: 16187
Mouse Il3 Predicted Functions
Enables cytokine activity; growth factor activity; and interleukin-3 receptor binding activity. Acts upstream of or within several processes, including cell surface receptor signaling pathway; monocyte differentiation; and regulation of phosphate metabolic process. Located in extracellular space. Is expressed in skin; stomach; thymus; and vascular system. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).
Rat Il3 Target Information
- Target Symbol: Il3, interleukin 3
- Alias:
- hematopoietic growth factor
- IL-3
- interleukin-3
- mast cell growth factor
- MCGF
- multipotential colony-stimulating factor
- P-cell-stimulating factor
- NCBI_Gene: 24495
Rat Il3 Predicted Functions
Enables cytokine activity and interleukin-3 receptor binding activity. Involved in several processes, including hemopoiesis; positive regulation of cell population proliferation; and regulation of cell growth. Located in extracellular space. Used to study osteosarcoma. Biomarker of prostatic hypertrophy and pyelonephritis. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).
