Human IL-3 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL3
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.94 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	87% – 119%
Average Recovery	1.01

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.94 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	87% – 119%
Average Recovery	1.01
Intra Precision	4.3%-9.5%
Inter-Precision	1.5% – 8.3%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-3 monoclonal antibody Human IL-3 freeze-dried standard IL-3 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL3

IL3 Target Infomation Overview

Target Symbol: IL3, interleukin 3
Gene Groups: Interleukins
Alias: IL-3; MULTI-CSF; MCGF; MGC79398; MGC79399
Alias Names: multilineage-colony-stimulating factor; hematopoietic growth factor; P-cell stimulating factor; mast-cell growth factor; colony-stimulating factor, multiple; interleukin 3 (colony-stimulating factor, multiple)

IL3, interleukin 3 Target Infomation by Species

HumanMouseRat

Human IL3 Target Information

Target Symbol: IL3, interleukin 3
Alias:
- colony-stimulating factor, multiple
- hematopoietic growth factor
- IL-3
- interleukin-3
- mast cell growth factor
- mast-cell growth factor
- MCGF
- MGC79398
- MGC79399
- MULTI-CSF
- multilineage-colony-stimulating factor
- multipotential colony-stimulating factor
- P-cell stimulating factor
- P-cell-stimulating factor
NCBI_Gene: 3562
UniProtKB: P08700

Human IL3 Predicted Functions

Predicted to enable interleukin-3 receptor binding activity. Involved in embryonic hemopoiesis; positive regulation of cell population proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Implicated in Graves’ disease; asthma; and rheumatoid arthritis. Biomarker of several diseases, including Ebola hemorrhagic fever; genital herpes; major depressive disorder; obesity; and psoriasis.

Mouse Il3 Target Information

Target Symbol: Il3, interleukin 3
Alias:
- BPA
- colony stimulating factor, multi (contains Il-3)
- Csfmu
- HCGF
- Il-3
- MCGF
- PSF
NCBI_Gene: 16187

Mouse Il3 Predicted Functions

Enables cytokine activity; growth factor activity; and interleukin-3 receptor binding activity. Acts upstream of or within several processes, including cell surface receptor signaling pathway; monocyte differentiation; and regulation of phosphate metabolic process. Located in extracellular space. Is expressed in skin; stomach; thymus; and vascular system. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).

Rat Il3 Target Information

Target Symbol: Il3, interleukin 3
Alias:
- hematopoietic growth factor
- IL-3
- interleukin-3
- mast cell growth factor
- MCGF
- multipotential colony-stimulating factor
- P-cell-stimulating factor
NCBI_Gene: 24495

Rat Il3 Predicted Functions

Enables cytokine activity and interleukin-3 receptor binding activity. Involved in several processes, including hemopoiesis; positive regulation of cell population proliferation; and regulation of cell growth. Located in extracellular space. Used to study osteosarcoma. Biomarker of prostatic hypertrophy and pyelonephritis. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).