Human IL-3 ELISA Kit

$350.00$450.00

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TARGETSIL3
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.94 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 119%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.94 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 119%
Average Recovery1.01
Intra Precision4.3%-9.5%
Inter-Precision1.5% – 8.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-3 monoclonal antibody
Human IL-3 freeze-dried standard
IL-3 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL3

IL3 Target Infomation Overview

  • Target Symbol: IL3, interleukin 3
  • Gene Groups: Interleukins
  • Alias: IL-3; MULTI-CSF; MCGF; MGC79398; MGC79399
  • Alias Names: multilineage-colony-stimulating factor; hematopoietic growth factor; P-cell stimulating factor; mast-cell growth factor; colony-stimulating factor, multiple; interleukin 3 (colony-stimulating factor, multiple)

IL3, interleukin 3 Target Infomation by Species

HumanMouseRat

Human IL3 Target Information

  • Target Symbol: IL3, interleukin 3
  • Alias:
    • colony-stimulating factor, multiple
    • hematopoietic growth factor
    • IL-3
    • interleukin-3
    • mast cell growth factor
    • mast-cell growth factor
    • MCGF
    • MGC79398
    • MGC79399
    • MULTI-CSF
    • multilineage-colony-stimulating factor
    • multipotential colony-stimulating factor
    • P-cell stimulating factor
    • P-cell-stimulating factor
  • NCBI_Gene: 3562
  • UniProtKB: P08700

Human IL3 Predicted Functions

Predicted to enable interleukin-3 receptor binding activity. Involved in embryonic hemopoiesis; positive regulation of cell population proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Implicated in Graves’ disease; asthma; and rheumatoid arthritis. Biomarker of several diseases, including Ebola hemorrhagic fever; genital herpes; major depressive disorder; obesity; and psoriasis.

Mouse Il3 Target Information

Mouse Il3 Predicted Functions

Enables cytokine activity; growth factor activity; and interleukin-3 receptor binding activity. Acts upstream of or within several processes, including cell surface receptor signaling pathway; monocyte differentiation; and regulation of phosphate metabolic process. Located in extracellular space. Is expressed in skin; stomach; thymus; and vascular system. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).

Rat Il3 Target Information

Rat Il3 Predicted Functions

Enables cytokine activity and interleukin-3 receptor binding activity. Involved in several processes, including hemopoiesis; positive regulation of cell population proliferation; and regulation of cell growth. Located in extracellular space. Used to study osteosarcoma. Biomarker of prostatic hypertrophy and pyelonephritis. Human ortholog(s) of this gene implicated in Graves’ disease; asthma; and rheumatoid arthritis. Orthologous to human IL3 (interleukin 3).

IL3 Target News

    Allergic Reaction
    Lung Disease (Asthma & COPD)
    Transplantation

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