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Human IL-31 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 50 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.78 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 84% – 117% |
| Average Recovery | 0.98 |
| Intra Precision | 4.3% – 5.7% |
| Inter-Precision | 4.0% – 4.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-31 monoclonal antibody Human IL-31 freeze-dried standard IL-31 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-31 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-31 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-31 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL31
IL31 Target Infomation Overview
- Target Symbol: IL31, interleukin 31
- Gene Groups: Interleukins
- Alias: IL-31
IL31, interleukin 31 Target Infomation by Species
HumanMouseRat
Human IL31 Target Information
- Target Symbol: IL31, interleukin 31
- Alias:
- IL-31
- interleukin-31
- NCBI_Gene: 386653
- UniProtKB: Q6EBC2
Human IL31 Predicted Functions
Predicted to enable cytokine activity and oncostatin-M receptor binding activity. Predicted to be involved in immune system process and signal transduction. Located in extracellular space.
Mouse Il31 Target Information
- Target Symbol: Il31, interleukin 31
- Alias:
- 1700013B14Rik
- RIKEN cDNA 1700013B14 gene
- NCBI_Gene: 76399
Mouse Il31 Predicted Functions
Enables cytokine activity and oncostatin-M receptor binding activity. Predicted to act upstream of or within immune system process. Located in extracellular space. Orthologous to human IL31 (interleukin 31).
Rat Il31 Target Information
- Target Symbol: Il31, interleukin 31
- Alias:
- hypothetical protein LOC690028
- interleukin-31
- LOC690028
- NCBI_Gene: 690028
- 暂无
Rat Il31 Predicted Functions
Predicted to enable cytokine activity and oncostatin-M receptor binding activity. Predicted to be located in extracellular space. Orthologous to human IL31 (interleukin 31).
