EK131

Human IL-31 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity0.78 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery84% – 117%
Average Recovery98%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity0.78 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery84% – 117%
Average Recovery98%
Intra Precision4.3% – 5.7%
Inter Precision4.0% – 4.2%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-31 monoclonal antibody
Human IL-31 freeze-dried standard
IL-31 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-31 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-31 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-31 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL31

IL31 Target Infomation Overview

  • Target Symbol: IL31, interleukin 31
  • Gene Groups: Interleukins
  • Alias: IL-31

IL31, interleukin 31 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL31 Target Information

Human IL31 Predicted Functions

Predicted to enable cytokine activity and oncostatin-M receptor binding activity. Predicted to be involved in immune system process and signal transduction. Located in extracellular space.

Mouse Il31 Target Information

Mouse Il31 Predicted Functions

Enables cytokine activity and oncostatin-M receptor binding activity. Predicted to act upstream of or within immune system process. Located in extracellular space. Orthologous to human IL31 (interleukin 31).

Rat Il31 Target Information

  • Target Symbol: Il31, interleukin 31
  • Alias:
    • hypothetical protein LOC690028
    • interleukin-31
    • LOC690028
  • NCBI_Gene: 690028
  • 暂无

Rat Il31 Predicted Functions

Predicted to enable cytokine activity and oncostatin-M receptor binding activity. Predicted to be located in extracellular space. Orthologous to human IL31 (interleukin 31).

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