Human KGF/FGF-7 ELISA Kit

$245.00 – $350.00

Related Target	FGF7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.05 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 120%
Average Recovery	96%

ELISA Kit Detail Information

Related Target	FGF7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.05 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 120%
Average Recovery	96%
Intra Precision	3.6% – 4.4%
Inter Precision	2.1% – 3.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-KGF monoclonal antibody Human KGF freeze-dried standard KGF detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human KGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, KGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of KGF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

FGF7

FGF7 Target Infomation Overview

Target Symbol: FGF7, fibroblast growth factor 7
Gene Groups: Receptor ligands; Fibroblast growth factor family
Alias: KGF
Alias Names: keratinocyte growth factor; fibroblast growth factor 7 (keratinocyte growth factor)

FGF7, fibroblast growth factor 7 Target Infomation by Species

Human
Mouse
Rat

Human FGF7 Target Information

Target Symbol: FGF7, fibroblast growth factor 7
Alias:
- FGF-7
- HBGF-7
- heparin-binding growth factor 7
- keratinocyte growth factor
- KGF
NCBI_Gene: 2252
UniProtKB: P21781

Human FGF7 Predicted Functions

Enables chemoattractant activity. Involved in several processes, including positive regulation of keratinocyte migration; positive regulation of keratinocyte proliferation; and protein localization to cell surface. Acts upstream of or within fibroblast growth factor receptor signaling pathway and positive regulation of epithelial cell proliferation involved in lung morphogenesis. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space. Biomarker of several diseases, including inflammatory bowel disease (multiple); leiomyoma; prostatic hypertrophy; reproductive organ cancer (multiple); and severe acute respiratory syndrome.

Mouse Fgf7 Target Information

Target Symbol: Fgf7, fibroblast growth factor 7
Alias:
- Keratinocyte growth factor
- Kgf
NCBI_Gene: 14178
- UniprotKB: P36363
- UniprotKB: Q544I6

Mouse Fgf7 Predicted Functions

Predicted to enable heparin binding activity; receptor ligand activity; and type 2 fibroblast growth factor receptor binding activity. Involved in positive regulation of transcription, DNA-templated. Acts upstream of or within morphogenesis of an epithelium; positive regulation of epithelial cell proliferation; and regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in Golgi apparatus and extracellular region. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; genitourinary system; and integumental system. Orthologous to human FGF7 (fibroblast growth factor 7).

Rat Fgf7 Target Information

Target Symbol: Fgf7, fibroblast growth factor 7
Alias:
- FGF-7
- Fgf5b
- fibroblast growth factor 5b
- HBGF-7
- heparin-binding growth factor 7
- keratinocyte growth factor
- Keratinocyte Growth Factor Fgf-7
- Kgf
- KGF/FGF7
NCBI_Gene: 29348
- UniprotKB: G3V775
- UniprotKB: Q02195

Rat Fgf7 Predicted Functions

Enables growth factor activity; heparin binding activity; and type 2 fibroblast growth factor receptor binding activity. Involved in several processes, including ovarian follicle development; phosphatidylcholine biosynthetic process; and surfactant homeostasis. Located in extracellular space. Orthologous to human FGF7 (fibroblast growth factor 7).

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