EK185

Human L-Selectin ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity9.46 pg/mL
Array Range78.13 ng/mL – 5000 ng/mL
Assay Time3.5 h
Recovery90% – 119%
Average Recovery104%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity9.46 pg/mL
Array Range78.13 ng/mL – 5000 ng/mL
Assay Time3.5 h
Recovery90% – 119%
Average Recovery104%
Intra Precision6.9% – 7.1%
Inter Precision1.7% – 7.3%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sL-Selectin monoclonal antibody
Human sL-Selectin freeze-dried standard
sL-Selectin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sL-Selectin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sL-Selectin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sL-Selectin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

SELL

SELL Target Infomation Overview

  • Target Symbol: SELL, selectin L
  • Gene Groups: CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
  • Alias: LSEL; LAM1; LAM-1; hLHRc; Leu-8; Lyam-1; PLNHR; CD62L
  • Previous Names: LYAM1; LNHR
  • Alias Names: lymphocyte adhesion molecule 1

SELL, selectin L Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human SELL Target Information

  • Target Symbol: SELL, selectin L
  • Alias:
    • CD62 antigen-like family member L
    • CD62L
    • gp90-MEL
    • hLHRc
    • L-selectin
    • LAM-1
    • LAM1
    • LECAM1
    • Leu-8
    • LEU8
    • leukocyte adhesion molecule 1
    • leukocyte surface antigen Leu-8
    • leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Lyam-1
    • LYAM1
    • lymph node homing receptor
    • lymphocyte adhesion molecule 1
    • pln homing receptor
    • PLNHR
    • TQ1
  • NCBI_Gene: 6402
  • UniProtKB: P14151

Human SELL Predicted Functions

Enables calcium ion binding activity; oligosaccharide binding activity; and protease binding activity. Involved in calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules and leukocyte tethering or rolling. Is integral component of plasma membrane. Implicated in Crohn’s disease; IgA glomerulonephritis; and ulcerative colitis. Biomarker of Alzheimer’s disease; IgA glomerulonephritis; and systemic lupus erythematosus.

Mouse Sell Target Information

Mouse Sell Predicted Functions

Enables cell adhesion molecule binding activity. Acts upstream of or within response to ATP. Located in external side of plasma membrane. Is expressed in brain; brainstem; submandibular gland epithelium; and testis. Used to study type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in Crohn’s disease; IgA glomerulonephritis; and ulcerative colitis. Orthologous to human SELL (selectin L).

Rat Sell Target Information

  • Target Symbol: Sell, selectin L
  • Alias:
    • A.11
    • CD62 antigen-like family member L
    • L-selectin
    • LAM-1
    • LECAM-1
    • LECAM1
    • leukocyte adhesion molecule 1
    • leukocyte-endothelial cell adhesion molecule 1
    • ly-22
    • lymph node homing receptor
    • lymphocyte antigen 22
    • lymphocyte surface MEL-14 antigen
    • selectin, lymphocyte
  • NCBI_Gene: 29259

Rat Sell Predicted Functions

Enables glycolipid binding activity. Involved in several processes, including positive regulation of neutrophil chemotaxis; response to fatty acid; and response to hyperoxia. Located in cell surface. Used to study colitis and peripheral vascular disease. Biomarker of abdominal aortic aneurysm; endometriosis; pancreatitis; and toxic shock syndrome. Human ortholog(s) of this gene implicated in Crohn’s disease; IgA glomerulonephritis; and ulcerative colitis. Orthologous to human SELL (selectin L).

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