Name: Human Lipocalin-2/NGAL ELISA Kit
SKU: EK1116
Availability: InStock

EK1116

Human Lipocalin-2/NGAL ELISA Kit

$245.00 – $350.00

Related Target	LCN2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	2.42 pg/mL
Array Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	84% – 107%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	LCN2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	2.42 pg/mL
Array Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	84% – 107%
Average Recovery	98%
Intra Precision	2.0% – 4.5%
Inter Precision	2.9% – 5.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Lipocalin-2/NGAL monoclonal antibody Human Lipocalin-2/NGAL freeze-dried standard Lipocalin-2/NGAL detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Lipocalin-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Lipocalin-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Lipocalin-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

LCN2

LCN2 Target Infomation Overview

Target Symbol: LCN2, lipocalin 2
Gene Groups: Lipocalins
Alias: NGAL; 24p3
Alias Names: oncogene 24p3; neutrophil gelatinase-associated lipocalin; siderocalin; lipocalin 2 (oncogene 24p3)

LCN2, lipocalin 2 Target Infomation by Species

Human
Mouse
Rat

Human LCN2 Target Information

Target Symbol: LCN2, lipocalin 2
Alias:
- 24p3
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
- lipocalin-2
- migration-stimulating factor inhibitor
- MSFI
- neutrophil gelatinase-associated lipocalin
- NGAL
- oncogene 24p3
- p25
- siderocalin
- siderocalin LCN2
NCBI_Gene: 3934
UniProtKB: P80188

Human LCN2 Predicted Functions

Enables enterobactin binding activity; identical protein binding activity; and iron ion binding activity. Involved in defense response to bacterium and sequestering of iron ion. Located in extracellular space. Implicated in urinary tract infection. Biomarker of several diseases, including congenital diaphragmatic hernia; hidradenitis suppurativa; hydronephrosis; hypertension (multiple); and kidney disease (multiple).

Mouse Lcn2 Target Information

Target Symbol: Lcn2, lipocalin 2
Alias:
- 24p3
- AW212229
- expressed sequence AW212229
- neu-related lipocalin
- NGAL
- NRL
NCBI_Gene: 16819
- UniprotKB: P11672
- UniprotKB: A0A0A6YW77

Mouse Lcn2 Predicted Functions

Enables iron ion binding activity. Involved in innate immune response; positive regulation of cold-induced thermogenesis; and siderophore transport. Acts upstream of or within extrinsic apoptotic signaling pathway in absence of ligand; response to bacterium; and response to virus. Located in extracellular space. Is expressed in several structures, including genitourinary system; gut; liver; lung; and spleen. Human ortholog(s) of this gene implicated in intrahepatic cholangiocarcinoma and urinary tract infection. Orthologous to human LCN2 (lipocalin 2).

Rat Lcn2 Target Information

Target Symbol: Lcn2, lipocalin 2
Alias:
- alpha-2-microglobulin-related protein
- alpha-2U globulin-related protein
- lipocalin 2 (oncogene 24p3)
- lipocalin 24p3
- lipocalin-2
- neutrophil gelatinase-associated lipocalin
- NGAL
- p25
- siderocalin LCN2
- Sip24
NCBI_Gene: 170496
UniProtKB: P30152

Rat Lcn2 Predicted Functions

Enables enterobactin binding activity; identical protein binding activity; and protease binding activity. Involved in several processes, including cellular response to cytokine stimulus; memory; and regulation of neuron apoptotic process. Located in cytosol and extracellular space. Used to study intrahepatic cholangiocarcinoma and retinal degeneration. Biomarker of several diseases, including acute kidney failure; congenital diaphragmatic hernia; obesity; pulmonary hypertension; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in urinary tract infection. Orthologous to human LCN2 (lipocalin 2).

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