Human MICB ELISA Kit

$350.00$450.00

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TARGETSMICB
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity53.2 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery76% – 125%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity53.2 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery76% – 125%
Average Recovery0.97
Intra Precision2.3% – 3.4%
Inter-Precision3.4%-4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- MICB monoclonal antibody
Human MICB freeze-dried standard
MICB detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MICB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MICB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MICB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MICB

MICB Target Infomation Overview

  • Target Symbol: MICB, MHC class I polypeptide-related sequence B
  • Gene Groups: C1-set domain containing
  • Alias: PERB11.2

MICB, MHC class I polypeptide-related sequence B Target Infomation by Species

HumanMouseRat

Human MICB Target Information

  • Target Symbol: MICB, MHC class I polypeptide-related sequence B
  • Alias:
    • MHC class I antigen-related protein B
    • MHC class I chain-related protein B
    • MHC class I mic-B antigen
    • MHC class I-like molecule PERB11.2-IMX
    • MIC-B
    • PERB11.2
    • stress inducible class I homolog
  • NCBI_Gene: 4277
  • UniProtKB: Q29980

Human MICB Predicted Functions

Enables natural killer cell lectin-like receptor binding activity. Involved in several processes, including immune response-activating cell surface receptor signaling pathway; negative regulation of defense response to virus by host; and response to retinoic acid. Predicted to be located in cell surface. Predicted to be anchored component of external side of plasma membrane. Predicted to be active in external side of plasma membrane and extracellular space.

Mouse Mill2 Target Information

Mouse Mill2 Predicted Functions

Predicted to enable natural killer cell lectin-like receptor binding activity. Predicted to be involved in immune response. Is anchored component of external side of plasma membrane. Orthologous to human MICA (MHC class I polypeptide-related sequence A) and MICB (MHC class I polypeptide-related sequence B).

Rat Micb Target Information

  • Target Symbol: Micb, MHC class I polypeptide-related sequence B
  • Alias:
    • MHC class I-like located near the leukocyte receptor complex 2
    • MHC class I-like located near the LRC, 2
    • MHC class I-like protein MILL2
    • MHC I like leukocyte 2
    • Mill2
    • Mill2 gene for MHC class I-like located near the LRC, 2, exon 3, partial cds, strain:LEW.1lm1
  • NCBI_Gene: 365212

Rat Micb Predicted Functions

Predicted to enable natural killer cell lectin-like receptor binding activity. Predicted to be involved in several processes, including immune response-activating cell surface receptor signaling pathway; negative regulation of defense response to virus by host; and response to retinoic acid. Predicted to be anchored component of membrane. Predicted to be active in external side of plasma membrane and extracellular space. Orthologous to human MICB (MHC class I polypeptide-related sequence B).

MICB Target News

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