Human MMP-10 ELISA Kit

$350.00$450.00

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TARGETSMMP10
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity4.27 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 122%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity4.27 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 122%
Average Recovery1.04
Intra Precision4.7% – 9.8%
Inter-Precision3.7% – 6.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-10 monoclonal antibody
Human MMP-10 freeze-dried standard
MMP-10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP10

MMP10 Target Infomation Overview

  • Target Symbol: MMP10, matrix metallopeptidase 10
  • Gene Groups: M10 matrix metallopeptidases
  • Previous Names: STMY2
  • Alias Names: matrix metalloproteinase 10 (stromelysin 2); stromelysin 2

MMP10, matrix metallopeptidase 10 Target Infomation by Species

HumanMouseRat

Human MMP10 Target Information

  • Target Symbol: MMP10, matrix metallopeptidase 10
  • Alias:
    • matrix metalloprotease 10
    • matrix metalloproteinase 10 (stromelysin 2)
    • matrix metalloproteinase-10
    • MMP-10
    • SL-2
    • STMY2
    • stromelysin 2
    • stromelysin-2
    • transin 2
    • transin-2
  • NCBI_Gene: 4319
  • UniProtKB: P09238

Human MMP10 Predicted Functions

Predicted to enable metalloendopeptidase activity. Predicted to be involved in collagen catabolic process and extracellular matrix organization. Predicted to act upstream of or within regulation of cell migration. Predicted to be located in extracellular space. Biomarker of adrenoleukodystrophy; diabetes mellitus; and end stage renal disease.

Mouse Mmp10 Target Information

Mouse Mmp10 Predicted Functions

Predicted to enable metalloendopeptidase activity. Acts upstream of or within regulation of cell migration. Predicted to be located in extracellular matrix and extracellular region. Is expressed in gonad; ovary; and testis. Orthologous to human MMP10 (matrix metallopeptidase 10).

Rat Mmp10 Target Information

  • Target Symbol: Mmp10, matrix metallopeptidase 10
  • Alias:
    • matrix metallopeptidase 10 (stromelysin 2)
    • matrix metalloproteinase 10
    • matrix metalloproteinase-10
    • MMP-10
    • SL-2
    • stromelysin 2
    • stromelysin-2
    • transformation-associated protein 34A
    • transin-2
  • NCBI_Gene: 117061

Rat Mmp10 Predicted Functions

Predicted to enable metalloendopeptidase activity. Predicted to be involved in collagen catabolic process and extracellular matrix organization. Predicted to act upstream of or within regulation of cell migration. Predicted to be located in extracellular matrix and extracellular region. Orthologous to human MMP10 (matrix metallopeptidase 10).

MMP10 Target News

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