EK1M02

Human MMP-2 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity69.14 pg/mL
Array Range2 ng/mL – 128 ng/mL
Assay Time3.5 h
Recovery86% – 118%
Average Recovery108%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity69.14 pg/mL
Array Range2 ng/mL – 128 ng/mL
Assay Time3.5 h
Recovery86% – 118%
Average Recovery108%
Intra Precision2.1% – 7.8%
Inter Precision2.4% – 3.8%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-2 monoclonal antibody
Human MMP-2 freeze-dried standard
MMP-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MMP2

MMP2 Target Infomation Overview

  • Target Symbol: MMP2, matrix metallopeptidase 2
  • Gene Groups: M10 matrix metallopeptidases
  • Alias: TBE-1
  • Previous Names: CLG4; CLG4A
  • Alias Names: matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

MMP2, matrix metallopeptidase 2 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human MMP2 Target Information

  • Target Symbol: MMP2, matrix metallopeptidase 2
  • Alias:
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG4
    • CLG4A
    • collagenase type IV-A
    • gelatinase A
    • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
    • matrix metalloproteinase-2
    • matrix metalloproteinase-II
    • MMP-2
    • MMP-II
    • MONA
    • neutrophil gelatinase
    • TBE-1
  • NCBI_Gene: 4313
  • UniProtKB: P08253

Human MMP2 Predicted Functions

Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).

Mouse Mmp2 Target Information

Mouse Mmp2 Predicted Functions

Enables metalloendopeptidase activity. Involved in response to amyloid-beta. Acts upstream of or within several processes, including blood vessel maturation; bone trabecula formation; and collagen catabolic process. Located in several cellular components, including extracellular space; mitochondrion; and sarcomere. Is expressed in several structures, including embryo mesenchyme; genitourinary system; heart; jaw; and musculoskeletal system. Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).

Rat Mmp2 Target Information

  • Target Symbol: Mmp2, matrix metallopeptidase 2
  • Alias:
    • 72 kDa gelatinase
    • 72 KDa type IV collagenase
    • gelatinase A
    • matrix metalloproteinase 2
    • matrix metalloproteinase 2 (72 KDa type IV collagenase)
    • matrix metalloproteinase-2
    • MMP-2
  • NCBI_Gene: 81686

Rat Mmp2 Predicted Functions

Enables fibronectin binding activity and peptidase activity. Involved in several processes, including cellular response to estradiol stimulus; circulatory system development; and reproductive structure development. Located in cytoplasm and extracellular space. Used to study artery disease (multiple); conjunctivitis; and transient cerebral ischemia. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); bile duct disease (multiple); brain ischemia (multiple); and liver cirrhosis (multiple). Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).

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