Human MMP-3 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 0.30 pg/mL |
| Array Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 105% |
| Average Recovery | 101% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 0.30 pg/mL |
| Array Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 105% |
| Average Recovery | 101% |
| Intra Precision | 4.0% – 4.9% |
| Inter Precision | 4.2% – 9.0% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-3 monoclonal antibody Human MMP-3 freeze-dried standard MMP-3 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-3 in the samples. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
MMP3
MMP3 Target Infomation Overview
- Target Symbol: MMP3, matrix metallopeptidase 3
- Gene Groups: M10 matrix metallopeptidases
- Previous Names: STMY1; STMY
- Alias Names: matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1
MMP3, matrix metallopeptidase 3 Target Infomation by Species
- Human
- Mouse
- Rat
Human MMP3 Target Information
- Target Symbol: MMP3, matrix metallopeptidase 3
- Alias:
- CHDS6
- matrix metallopeptidase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase-3
- MGC126102
- MGC126103
- MGC126104
- MMP-3
- proteoglycanase
- SL-1
- STMY
- STMY1
- STR1
- stromelysin-1
- transin-1
- NCBI_Gene: 4314
- UniProtKB: P08254
Human MMP3 Predicted Functions
Enables endopeptidase activity and metallopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of hydrogen peroxide metabolic process; and positive regulation of protein-containing complex assembly. Predicted to be located in cytosol and mitochondrion. Implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Biomarker of several diseases, including Takayasu’s arteritis; arthritis (multiple); autoimmune disease (multiple); conjunctivochalasis; and lung disease (multiple).
Mouse Mmp3 Target Information
- Target Symbol: Mmp3, matrix metallopeptidase 3
- Alias:
- progelatinase
- SLN-1
- SLN1
- Stmy1
- STR-1
- Str1
- stromelysin 1
- stromelysin-1
- NCBI_Gene: 17392
Mouse Mmp3 Predicted Functions
Predicted to enable metalloendopeptidase activity. Involved in several processes, including negative regulation of protein kinase B signaling; positive regulation of oxidative stress-induced cell death; and protein catabolic process. Acts upstream of or within cellular response to amino acid stimulus and regulation of cell migration. Located in cytosol and mitochondrion. Is expressed in several structures, including axial skeleton thoracic region; genitourinary system; liver; oral region epithelium; and palatal shelf. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Orthologous to human MMP3 (matrix metallopeptidase 3).
Rat Mmp3 Target Information
- Target Symbol: Mmp3, matrix metallopeptidase 3
- Alias:
- matrix metalloproteinase 3
- matrix metalloproteinase-3
- MMP-3
- PTR1 protein
- SL-1
- stromelysin-1
- transin-1
- NCBI_Gene: 171045
Rat Mmp3 Predicted Functions
Predicted to enable metalloendopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to interleukin-1; and response to estradiol. Located in several cellular components, including cell body; dendrite; and extracellular space. Part of protein-containing complex. Biomarker of several diseases, including abdominal aortic aneurysm; degenerative disc disease; gastric ulcer; glomerulonephritis (multiple); and primary biliary cholangitis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Orthologous to human MMP3 (matrix metallopeptidase 3).
Products with the Same Target - MMP3
Citations for Human MMP-3 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK406
- ELISA Kit
Guinea Pig IL-6 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1218
- ELISA Kit
Human Apolipoprotein E/Apo E ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1223
- ELISA Kit
Human Apolipoprotein D/Apo D ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1120
- ELISA Kit
Human Activin A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1258
- ELISA Kit
Human Apolipoprotein AII/Apo A2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK8100
- ELISA Kit
Cortisol Competitive ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.