Human MMP-7 ELISA Kit

$245.00 – $350.00

Related Target	MMP7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.90 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	82% – 104%
Average Recovery	92%

ELISA Kit Detail Information

Related Target	MMP7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.90 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	82% – 104%
Average Recovery	92%
Intra Precision	3.2% – 4.2%
Inter Precision	3.5% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-7 monoclonal antibody Human MMP-7 freeze-dried standard MMP-7 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MMP7

MMP7 Target Infomation Overview

Target Symbol: MMP7, matrix metallopeptidase 7
Gene Groups: M10 matrix metallopeptidases
Alias: PUMP-1
Previous Names: MPSL1
Alias Names: matrilysin; matrix metalloproteinase 7 (matrilysin, uterine)

MMP7, matrix metallopeptidase 7 Target Infomation by Species

Human
Mouse
Rat

Human MMP7 Target Information

Target Symbol: MMP7, matrix metallopeptidase 7
Alias:
- matrilysin
- matrin
- matrix metallopeptidase 7 (matrilysin, uterine)
- matrix metalloproteinase 7
- matrix metalloproteinase 7 (matrilysin, uterine)
- matrix metalloproteinase-7
- MMP-7
- MPSL1
- PUMP-1
- pump-1 protease
- uterine matrilysin
- uterine metalloproteinase
NCBI_Gene: 4316
UniProtKB: P09237

Human MMP7 Predicted Functions

Enables endopeptidase activity and metallopeptidase activity. Involved in positive regulation of cell migration and proteolysis. Acts upstream of or within membrane protein ectodomain proteolysis and membrane protein intracellular domain proteolysis. Located in extracellular exosome. Biomarker of endometrial cancer; endometrial hyperplasia; glioblastoma; and lung non-small cell carcinoma.

Mouse Mmp7 Target Information

Target Symbol: Mmp7, matrix metallopeptidase 7
Alias:
- MAT
- matrilysin
NCBI_Gene: 17393

Mouse Mmp7 Predicted Functions

Enables peptidase activity. Acts upstream of or within several processes, including defense response to bacterium; proteolysis; and response to xenobiotic stimulus. Predicted to be located in cell surface. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; hemolymphoid system gland; integumental system; liver and biliary system; and reproductive system. Orthologous to human MMP7 (matrix metallopeptidase 7).

Rat Mmp7 Target Information

Target Symbol: Mmp7, matrix metallopeptidase 7
Alias:
- matrilysin
- matrin
- matrix metalloproteinase 7
- Matrix metalloproteinase 7 (matrilysin)
- matrix metalloproteinase-7
- MMP-7
- MPMM
- pump-1 protease
- uterine metalloproteinase
NCBI_Gene: 25335
UniProtKB: P50280

Rat Mmp7 Predicted Functions

Enables heparin binding activity. Involved in several processes, including cellular response to mechanical stimulus; estrous cycle; and maternal process involved in female pregnancy. Located in cell surface and extracellular space. Used to study hypertension and prostate carcinoma. Biomarker of bladder neck obstruction; coronary restenosis; hypertension; prostate cancer; and renal hypertension. Orthologous to human MMP7 (matrix metallopeptidase 7).

Citations for Human MMP-7 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.