Human MMP-8 ELISA Kit

$245.00 – $350.00

Related Target	MMP8
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	12.66 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	88% – 107%
Average Recovery	97%

ELISA Kit Detail Information

Related Target	MMP8
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	12.66 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	88% – 107%
Average Recovery	97%
Intra Precision	3.1% – 4.5%
Inter Precision	4.6% – 7.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-8 monoclonal antibody Human MMP-8 freeze-dried standard MMP-8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-8 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-8 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-8 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MMP8

MMP8 Target Infomation Overview

Target Symbol: MMP8, matrix metallopeptidase 8
Gene Groups: M10 matrix metallopeptidases
Previous Names: CLG1
Alias Names: matrix metalloproteinase 8 (neutrophil collagenase)

MMP8, matrix metallopeptidase 8 Target Infomation by Species

Human
Mouse
Rat

Human MMP8 Target Information

Target Symbol: MMP8, matrix metallopeptidase 8
Alias:
- CLG1
- collagenase 2
- HNC
- matrix metalloproteinase 8 (neutrophil collagenase)
- matrix metalloproteinase-8
- MMP-8
- neutrophil collagenase
- PMN leukocyte collagenase
- PMNL collagenase
- PMNL-CL
NCBI_Gene: 4317
UniProtKB: P22894

Human MMP8 Predicted Functions

Enables serine-type endopeptidase activity. Involved in endodermal cell differentiation and proteolysis. Located in extracellular space. Implicated in breast cancer and carotid artery disease. Biomarker of COVID-19; aortic aneurysm (multiple); arthritis (multiple); breast cancer; and female reproductive organ cancer (multiple).

Mouse Mmp8 Target Information

Target Symbol: Mmp8, matrix metallopeptidase 8
Alias:
- BB138268
- Collagenase-2
- expressed sequence BB138268
NCBI_Gene: 17394

Mouse Mmp8 Predicted Functions

Predicted to enable endopeptidase activity and metal ion binding activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of intracellular signal transduction; and regulation of cytokine production. Located in extracellular space. Is expressed in several structures, including central nervous system; genitourinary system; hindlimb digit mesenchyme; musculature; and skeleton. Human ortholog(s) of this gene implicated in breast cancer and carotid artery disease. Orthologous to human MMP8 (matrix metallopeptidase 8).

Rat Mmp8 Target Information

Target Symbol: Mmp8, matrix metallopeptidase 8
Alias:
- matrix metalloproteinase-8
- MMP-8
- neutrophil collagenase
- neutrophil collagenease
NCBI_Gene: 63849
- UniprotKB: G3V7D0
- UniprotKB: O88766

Rat Mmp8 Predicted Functions

Enables calcium ion binding activity; metallopeptidase activity; and zinc ion binding activity. Involved in several processes, including positive regulation of neuroinflammatory response; positive regulation of nitric oxide biosynthetic process; and positive regulation of tumor necrosis factor production. Located in extracellular space. Biomarker of congestive heart failure. Human ortholog(s) of this gene implicated in breast cancer and carotid artery disease. Orthologous to human MMP8 (matrix metallopeptidase 8).

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