Human/Mouse/Rat TGF-β1 ELISA Kit

$350.00$450.00

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TARGETSTGFB1
SPECIESHuman Mouse Rat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.36 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 117%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.36 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 117%
Average Recovery0.99
Intra Precision2.0% – 5.3%
Inter-Precision4.1% – 6.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β1A monoclonal antibody
Human /Mouse /Rat TGF-β1A freeze-dried standard
TGF-β1A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human /mouse /rat TGF-β1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TGF-β1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TGF-β1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TGFB1

TGFB1 Target Infomation Overview

  • Target Symbol: TGFB1, transforming growth factor beta 1
  • Gene Groups: Transforming growth factor beta family
  • Alias: CED; TGFbeta
  • Previous Names: TGFB; DPD1
  • Alias Names: Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1, transforming growth factor beta 1 Target Infomation by Species

HumanMouseRat

Human TGFB1 Target Information

  • Target Symbol: TGFB1, transforming growth factor beta 1
  • Alias:
    • CED
    • DPD1
    • IBDIMDE
    • LAP
    • latency-associated peptide
    • prepro-transforming growth factor beta-1
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta1
    • TGFB
    • TGFbeta
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor beta1
  • NCBI_Gene: 7040
  • UniProtKB: P01137

Human TGFB1 Predicted Functions

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

Mouse Tgfb1 Target Information

Mouse Tgfb1 Predicted Functions

Enables protein serine/threonine kinase activator activity and transforming growth factor beta receptor binding activity. Involved in several processes, including circulatory system development; positive regulation of leukocyte differentiation; and regulation of cytokine production. Acts upstream of or within several processes, including animal organ development; positive regulation of histone modification; and regulation of protein localization to nucleus. Located in cytoplasm; extracellular matrix; and extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; genitourinary system; heart; and skin. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).

Rat Tgfb1 Target Information

  • Target Symbol: Tgfb1, transforming growth factor, beta 1
  • Alias:
    • TGF-beta-1
    • Tgfb
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor, beta-1
  • NCBI_Gene: 59086
  • UniProtKB: P17246

Rat Tgfb1 Predicted Functions

Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; cellular response to hormone stimulus; and regulation of epithelial cell proliferation. Acts upstream of or within positive regulation of smooth muscle cell differentiation. Located in several cellular components, including cell surface; neuronal cell body; and secretory granule. Used to study chronic obstructive pulmonary disease; familial hyperlipidemia; hypertension; proteinuria; and pulmonary fibrosis. Biomarker of several diseases, including allergic rhinitis; artery disease (multiple); cerebrovascular disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).

TGFB1 Target News

    3.7
    Blocking BAFF Alleviates Hepatic Fibrosis in Schistosoma japonicum-Infected Mice
    Pathogens
    5.9
    Potent Intestinal Mucosal Barrier Enhancement of Nostoc commune Vaucher Polysaccharide Supplementation Ameliorates Acute Ulcerative Colitis in Mice Mediated by Gut Microbiota
    Nutrients
    5.6
    Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    1.798
    Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation
    EXPERIMENTAL ANIMALS
    3.7
    DNMT3A-mediated epigenetic silencing of SOX17 contributes to endothelial cell migration and fibroblast activation in wound healing
    PLoS One
    3.6
    IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor Microenvironment
    Journal of Clinical and Translational Hepatology
    12.658
    Procoxacin bidirectionally inhibits osteoblastic and osteoclastic activity in bone and suppresses bone metastasis of prostate cancer
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
    10.2
    Synergetic regulation of cancer cells and exhausted T cells to fight cold tumors with a fluorinated EGCG-based nanocomplex
    JOURNAL OF NANOBIOTECHNOLOGY
    10.2
    Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA
    JOURNAL OF NANOBIOTECHNOLOGY
    37.3
    Hsa_circ_0136666 stimulates gastric cancer progression and tumor immune escape by regulating the miR-375/PRKDC Axis and PD-L1 phosphorylation
    Molecular Cancer
    14.957
    Adoptive Treg therapy with metabolic intervention via perforated microneedles ameliorates psoriasis syndrome
    Science Advances
    11.061
    3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis
    Biofabrication
    3.889
    Panax notoginseng saponins (PNS) attenuate Th17 cell differentiation in CIA mice via inhibition of nuclear PKM2-mediated STAT3 phosphorylation
    PHARMACEUTICAL BIOLOGY
    1.8
    Chloroquine Alleviates Atherosclerosis by Modulating Regulatory T Cells Through the ATM/AMPK/mTOR Signaling Pathway in ApoE−/− Mice
    EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES
    4.6
    Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization
    Scientific Reports
    17.694
    Temperature sensitive liposome based cancer nanomedicine enables tumour lymph node immune microenvironment remodelling
    Nature Communications
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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