Human/Mouse TGF-β1 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	TGFB1
SPECIES	Human Mouse Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	3.36 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 117%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	3.36 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 117%
Average Recovery	0.99
Intra Precision	2.0% – 5.3%
Inter-Precision	4.1% – 6.0%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β1A monoclonal antibody Human /Mouse /Rat TGF-β1A freeze-dried standard TGF-β1A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human /mouse /rat TGF-β1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TGF-β1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TGF-β1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TGFB1

TGFB1 Target Infomation Overview

Target Symbol: TGFB1, transforming growth factor beta 1
Gene Groups: Transforming growth factor beta family
Alias: CED; TGFbeta
Previous Names: TGFB; DPD1
Alias Names: Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1, transforming growth factor beta 1 Target Infomation by Species

HumanMouseRat

Human TGFB1 Target Information

Target Symbol: TGFB1, transforming growth factor beta 1
Alias:
- CED
- DPD1
- IBDIMDE
- LAP
- latency-associated peptide
- prepro-transforming growth factor beta-1
- TGF-beta 1 protein
- TGF-beta-1
- TGF-beta1
- TGFB
- TGFbeta
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor beta1
NCBI_Gene: 7040
UniProtKB: P01137

Human TGFB1 Predicted Functions

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

Mouse Tgfb1 Target Information

Target Symbol: Tgfb1, transforming growth factor, beta 1
Alias:
- TGF-beta 1
- TGF-beta1
- Tgfb
- Tgfb-1
- TGFbeta1
NCBI_Gene: 21803

Mouse Tgfb1 Predicted Functions

Enables protein serine/threonine kinase activator activity and transforming growth factor beta receptor binding activity. Involved in several processes, including circulatory system development; positive regulation of leukocyte differentiation; and regulation of cytokine production. Acts upstream of or within several processes, including animal organ development; positive regulation of histone modification; and regulation of protein localization to nucleus. Located in cytoplasm; extracellular matrix; and extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; genitourinary system; heart; and skin. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).

Rat Tgfb1 Target Information

Target Symbol: Tgfb1, transforming growth factor, beta 1
Alias:
- TGF-beta-1
- Tgfb
- transforming growth factor beta-1
- transforming growth factor beta-1 proprotein
- transforming growth factor, beta-1
NCBI_Gene: 59086
UniProtKB: P17246

Rat Tgfb1 Predicted Functions

Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; cellular response to hormone stimulus; and regulation of epithelial cell proliferation. Acts upstream of or within positive regulation of smooth muscle cell differentiation. Located in several cellular components, including cell surface; neuronal cell body; and secretory granule. Used to study chronic obstructive pulmonary disease; familial hyperlipidemia; hypertension; proteinuria; and pulmonary fibrosis. Biomarker of several diseases, including allergic rhinitis; artery disease (multiple); cerebrovascular disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Orthologous to human TGFB1 (transforming growth factor beta 1).