Human MSP/MST1 ELISA Kit

$245.00 – $350.00

Related Target	MST1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.33 pg/mL
Array Range	39.06 pg/mL – 2500 pg/mL
Assay Time	3.5 h
Recovery	80% – 126%
Average Recovery	104%

ELISA Kit Detail Information

Related Target	MST1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.33 pg/mL
Array Range	39.06 pg/mL – 2500 pg/mL
Assay Time	3.5 h
Recovery	80% – 126%
Average Recovery	104%
Intra Precision	1.6% – 3.4%
Inter Precision	2.5% - 3.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MSP monoclonal antibody Human MSP freeze-dried standard Human MSP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MSP antibodies are precoated on a high-affinity ELISA plate.Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MSP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MSP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MST1

MST1 Target Infomation Overview

Target Symbol: MST1, macrophage stimulating 1
Alias: MSP; NF15S2
Previous Names: D3F15S2; HGFL; DNF15S2
Alias Names: hepatocyte growth factor-like

MST1, macrophage stimulating 1 Target Infomation by Species

Human
Mouse
Rat

Human MST1 Target Information

Target Symbol: MST1, macrophage stimulating 1
Alias:
- D3F15S2
- DNF15S2
- hepatocyte growth factor-like protein
- hepatocyte growth factor-like protein homolog
- HGFL
- macrophage stimulating 1 (hepatocyte growth factor-like)
- macrophage stimulatory protein
- macrophage-stimulating protein
- MSP
- NF15S2
NCBI_Gene: 4485
UniProtKB: P26927

Human MST1 Predicted Functions

Enables receptor tyrosine kinase binding activity. Involved in histone H2A-S139 phosphorylation; negative regulation of gluconeogenesis; and regulation of cAMP-dependent protein kinase activity. Located in extracellular space. Part of collagen-containing extracellular matrix.

Mouse Mst1 Target Information

Target Symbol: Mst1, macrophage stimulating 1 (hepatocyte growth factor-like)
Alias:
- D3F15S2h
- D9H3F15S2
- DNA segment, Chr 9, human D3F15S2
- DNF15S2h
- hepatocyte growth factor-like
- Hgfl
NCBI_Gene: 15235

Mouse Mst1 Predicted Functions

Predicted to enable receptor tyrosine kinase binding activity. Acts upstream of or within several processes, including embryo implantation; mammary duct terminal end bud growth; and positive regulation of mammary gland epithelial cell proliferation. Located in vacuole. Is expressed in esophagus epithelium; liver; liver parenchyma; pharynx epithelium; and stomach proventricular region epithelium. Orthologous to several human genes including MST1 (macrophage stimulating 1).

Rat Mst1 Target Information

Target Symbol: Mst1, macrophage stimulating 1
Alias:
- D8h3f15s2
- E2F transcription factor 2
- E2F2
- hepatocyte growth factor-like protein
- Macrophage stimulating 1 (hepatocyte growth factor-like)
NCBI_Gene: 24566
- UniprotKB: F7FMS0
- UniprotKB: P70521

Rat Mst1 Predicted Functions

Enables enzyme binding activity. Involved in cellular response to hypoxia; flagellated sperm motility; and spermatogenesis. Located in cytoplasm. Orthologous to several human genes including MST1 (macrophage stimulating 1).

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