Human Notch-1 ELISA Kit

$350.00$450.00

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TARGETSNOTCH1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity3.38 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery82% – 118%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity3.38 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery82% – 118%
Average Recovery0.98
Intra Precision2.0% – 3.3%
Inter-Precision2.7%-4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Notch-1 monoclonal antibody
Human Notch-1 freeze-dried standard
Notch-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Notch-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Notch-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Notch-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

NOTCH1

NOTCH1 Target Infomation Overview

  • Target Symbol: NOTCH1, notch receptor 1
  • Gene Groups: Notch receptors; MicroRNA protein coding host genes
  • Previous Names: TAN1
  • Alias Names: Notch (Drosophila) homolog 1 (translocation-associated); Notch homolog 1, translocation-associated (Drosophila); notch 1

NOTCH1, notch receptor 1 Target Infomation by Species

HumanMouseRat

Human NOTCH1 Target Information

  • Target Symbol: NOTCH1, notch receptor 1
  • Alias:
    • AOS5
    • AOVD1
    • hN1
    • neurogenic locus notch homolog protein 1
    • notch 1
    • notch gene homolog 1, (drosophila)
    • Notch homolog 1, translocation-associated
    • TAN1
    • translocation-associated notch protein TAN-1
  • NCBI_Gene: 4851
  • UniProtKB: P46531

Human NOTCH1 Predicted Functions

Enables identical protein binding activity. Involved in several processes, including heart development; negative regulation of endothelial cell migration; and positive regulation of intracellular signal transduction. Part of MAML1-RBP-Jkappa- ICN1 complex and receptor complex. Implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Biomarker of cholangiocarcinoma and endometrial cancer.

Mouse Notch1 Target Information

Mouse Notch1 Predicted Functions

Enables several functions, including Notch binding activity; chromatin DNA binding activity; and transcription coactivator activity. Involved in several processes, including blood vessel endothelial cell differentiation; heart development; and negative regulation of cell differentiation. Acts upstream of or within several processes, including animal organ development; nervous system development; and tube morphogenesis. Located in several cellular components, including adherens junction; apical plasma membrane; and cell surface. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Orthologous to human NOTCH1 (notch receptor 1).

Rat Notch1 Target Information

  • Target Symbol: Notch1, notch receptor 1
  • Alias:
    • Drosophila Notch homolog 1
    • Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila)
    • neurogenic locus notch homolog protein 1
    • NOTCH
    • notch 1
    • Notch gene homolog 1
    • Notch gene homolog 1 (Drosophila)
    • Notch homolog 1, translocation-associated
    • Notch homolog 1, translocation-associated (Drosophila)
    • TAN1
  • NCBI_Gene: 25496
  • UniProtKB: Q07008

Rat Notch1 Predicted Functions

Enables transmembrane signaling receptor activity. Involved in several processes, including nervous system development; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Located in several cellular components, including Golgi apparatus; acrosomal vesicle; and nucleus. Human ortholog(s) of this gene implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Orthologous to human NOTCH1 (notch receptor 1).

NOTCH1 Target News

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