Human Notch-1 ELISA Kit

$245.00 – $350.00

Related Target	NOTCH1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	3.38 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	82% – 118%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	NOTCH1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	3.38 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	82% – 118%
Average Recovery	98%
Intra Precision	2.0% – 3.3%
Inter Precision	2.7% - 4.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Notch-1 monoclonal antibody Human Notch-1 freeze-dried standard Notch-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Notch-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Notch-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Notch-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

NOTCH1

NOTCH1 Target Infomation Overview

Target Symbol: NOTCH1, notch receptor 1
Gene Groups: Notch receptors; MicroRNA protein coding host genes
Previous Names: TAN1
Alias Names: Notch (Drosophila) homolog 1 (translocation-associated); Notch homolog 1, translocation-associated (Drosophila); notch 1

NOTCH1, notch receptor 1 Target Infomation by Species

Human
Mouse
Rat

Human NOTCH1 Target Information

Target Symbol: NOTCH1, notch receptor 1
Alias:
- AOS5
- AOVD1
- hN1
- neurogenic locus notch homolog protein 1
- notch 1
- notch gene homolog 1, (drosophila)
- Notch homolog 1, translocation-associated
- TAN1
- translocation-associated notch protein TAN-1
NCBI_Gene: 4851
UniProtKB: P46531

Human NOTCH1 Predicted Functions

Enables identical protein binding activity. Involved in several processes, including heart development; negative regulation of endothelial cell migration; and positive regulation of intracellular signal transduction. Part of MAML1-RBP-Jkappa- ICN1 complex and receptor complex. Implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Biomarker of cholangiocarcinoma and endometrial cancer.

Mouse Notch1 Target Information

Target Symbol: Notch1, notch 1
Alias:
- 9930111A19Rik
- lin-12
- Mis6
- Motch A
- N1
- RIKEN cDNA 9930111A19 gene
- Tan1
- translocation-associated Notch
NCBI_Gene: 18128

Mouse Notch1 Predicted Functions

Enables several functions, including Notch binding activity; chromatin DNA binding activity; and transcription coactivator activity. Involved in several processes, including blood vessel endothelial cell differentiation; heart development; and negative regulation of cell differentiation. Acts upstream of or within several processes, including animal organ development; nervous system development; and tube morphogenesis. Located in several cellular components, including adherens junction; apical plasma membrane; and cell surface. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Orthologous to human NOTCH1 (notch receptor 1).

Rat Notch1 Target Information

Target Symbol: Notch1, notch receptor 1
Alias:
- Drosophila Notch homolog 1
- Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila)
- neurogenic locus notch homolog protein 1
- NOTCH
- notch 1
- Notch gene homolog 1
- Notch gene homolog 1 (Drosophila)
- Notch homolog 1, translocation-associated
- Notch homolog 1, translocation-associated (Drosophila)
- TAN1
NCBI_Gene: 25496
UniProtKB: Q07008

Rat Notch1 Predicted Functions

Enables transmembrane signaling receptor activity. Involved in several processes, including nervous system development; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Located in several cellular components, including Golgi apparatus; acrosomal vesicle; and nucleus. Human ortholog(s) of this gene implicated in Adams-Oliver syndrome; adult T-cell leukemia; and aortic valve disease 1. Orthologous to human NOTCH1 (notch receptor 1).

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