Human Proprotein Convertase 9 /PCSK9 ELISA Kit

$350.00$450.00

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TARGETSPCSK9
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity3.39 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery94% – 110%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity3.39 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery94% – 110%
Average Recovery1.03
Intra Precision1.7% – 7.6%
Inter-Precision2.4% – 5.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PCSK9 monoclonal antibody
Human PCSK9 freeze-dried standard
PCSK9 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PCSK9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PCSK9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PCSK9 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PCSK9

PCSK9 Target Infomation Overview

  • Target Symbol: PCSK9, proprotein convertase subtilisin/kexin type 9
  • Gene Groups: Proprotein convertase subtilisin/kexin family
  • Alias: NARC-1; FH3
  • Previous Names: HCHOLA3
  • Alias Names: hypercholesterolemia, autosomal dominant 3

PCSK9, proprotein convertase subtilisin/kexin type 9 Target Infomation by Species

HumanMouseRat

Human PCSK9 Target Information

  • Target Symbol: PCSK9, proprotein convertase subtilisin/kexin type 9
  • Alias:
    • convertase subtilisin/kexin type 9 preproprotein
    • FH3
    • FHCL3
    • HCHOLA3
    • LDLCQ1
    • NARC-1
    • NARC1
    • neural apoptosis regulated convertase 1
    • neural apoptosis-regulated convertase 1
    • PC9
    • proprotein convertase 9
    • subtilisin/kexin-like protease PC9
  • NCBI_Gene: 255738
  • UniProtKB: Q8NBP7

Human PCSK9 Predicted Functions

Enables several functions, including lipoprotein particle receptor binding activity; serine-type endopeptidase activity; and sodium channel inhibitor activity. Involved in several processes, including cholesterol homeostasis; low-density lipoprotein particle receptor catabolic process; and regulation of receptor-mediated endocytosis. Located in several cellular components, including Golgi apparatus; endosome; and perinuclear region of cytoplasm. Part of PCSK9-AnxA2 complex and PCSK9-LDLR complex. Implicated in familial hypercholesterolemia and hypobetalipoproteinemia.

Mouse Pcsk9 Target Information

  • Target Symbol: Pcsk9, proprotein convertase subtilisin/kexin type 9
  • Alias:
    • AI415265
    • AI747682
    • expressed sequence AI415265
    • expressed sequence AI747682
    • Narc1
  • NCBI_Gene: 100102

Mouse Pcsk9 Predicted Functions

Enables apolipoprotein binding activity; lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including cellular response to insulin stimulus; cellular response to starvation; and regulation of neuron apoptotic process. Acts upstream of or within several processes, including low-density lipoprotein receptor particle metabolic process; regulation of low-density lipoprotein particle receptor catabolic process; and triglyceride metabolic process. Located in COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum; and extracellular space. Is expressed in several structures, including alimentary system; cerebellum; genitourinary system; liver; and telencephalon. Human ortholog(s) of this gene implicated in familial hypercholesterolemia and hypobetalipoproteinemia. Orthologous to human PCSK9 (proprotein convertase subtilisin/kexin type 9).

Rat Pcsk9 Target Information

  • Target Symbol: Pcsk9, proprotein convertase subtilisin/kexin type 9
  • Alias:
    • NARC-1
    • Narc1
    • neural apoptosis regulated convertase 1
    • neural apoptosis-regulated convertase 1
    • PC9
    • proprotein convertase 9
    • proprotein convertase PC9
    • subtilisin/kexin-like protease PC9
  • NCBI_Gene: 298296
  • UniProtKB: P59996

Rat Pcsk9 Predicted Functions

Predicted to enable several functions, including lipoprotein particle binding activity; lipoprotein particle receptor binding activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to insulin stimulus; cholesterol homeostasis; and protein autoprocessing. Located in extracellular space and rough endoplasmic reticulum. Human ortholog(s) of this gene implicated in familial hypercholesterolemia and hypobetalipoproteinemia. Orthologous to human PCSK9 (proprotein convertase subtilisin/kexin type 9).

PCSK9 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)

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