Human RAGE ELISA Kit
$350.00 – $450.00
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
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Sample Volume | 20 μL |
Sensitivity | 0.75 pg/mL |
Range | 15.63 pg/mL – 1000 pg/mL |
Assay Time | 3.5 h |
Recovery | 89% – 108% |
Average Recovery | 1 |
Intra Precision | 3.4% – 5.0% |
Inter-Precision | 5.2% – 5.9% |
Platform | ELISA |
Plate | Detachable 96-well plate |
Size | 96T/48T |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RAGE monoclonal antibody Human RAGE freeze-dried standard RAGE detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RAGE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RAGE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RAGE in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
MOK
MOK Target Infomation Overview
- Target Symbol: MOK, MOK protein kinase
- Alias: RAGE1; STK30
- Previous Names: RAGE
- Alias Names: renal tumor antigen
MOK, MOK protein kinase Target Infomation by Species
Human MOK Target Information
- Target Symbol: MOK, MOK protein kinase
- Alias:
- MAPK/MAK/MRK overlapping kinase
- RAGE
- RAGE-1
- RAGE1
- renal cell carcinoma antigen
- renal cell carcinoma antigen (MOK protein kinase)
- renal tumor antigen 1
- STK30
- NCBI_Gene: 5891
- UniProtKB: Q9UQ07
Human MOK Predicted Functions
Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in ciliary base. Predicted to be active in cytoplasm and nucleus. Biomarker of renal cell carcinoma.
Mouse Mok Target Information
- Target Symbol: Mok, MOK protein kinase
- Alias:
- MAPK/MAK/MRK/ overlapping kinase
- MOK
- Rage
- renal tumor antigen
- serine/threonine kinase 30
- Stk30
- NCBI_Gene: 26448
Mouse Mok Predicted Functions
Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to act upstream of or within phosphorylation. Located in ciliary base and nucleus. Is expressed in gonad. Orthologous to human MOK (MOK protein kinase).
Rat Mok Target Information
- Target Symbol: Mok, MOK protein kinase
- Alias:
- LOC362787
- MAPK/MAK/MRK overlapping kinase
- MGC94988
- Rage
- renal tumor antigen
- NCBI_Gene: 362787
Rat Mok Predicted Functions
Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in ciliary base. Predicted to be active in cytoplasm and nucleus. Orthologous to human MOK (MOK protein kinase).