Human RAGE ELISA Kit

$350.00$450.00

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TARGETSMOK
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.75 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery89% – 108%
Average Recovery1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.75 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery89% – 108%
Average Recovery1
Intra Precision3.4% – 5.0%
Inter-Precision5.2% – 5.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RAGE monoclonal antibody
Human RAGE freeze-dried standard
RAGE detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RAGE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RAGE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RAGE in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MOK

MOK Target Infomation Overview

  • Target Symbol: MOK, MOK protein kinase
  • Alias: RAGE1; STK30
  • Previous Names: RAGE
  • Alias Names: renal tumor antigen

MOK, MOK protein kinase Target Infomation by Species

HumanMouseRat

Human MOK Target Information

  • Target Symbol: MOK, MOK protein kinase
  • Alias:
    • MAPK/MAK/MRK overlapping kinase
    • RAGE
    • RAGE-1
    • RAGE1
    • renal cell carcinoma antigen
    • renal cell carcinoma antigen (MOK protein kinase)
    • renal tumor antigen 1
    • STK30
  • NCBI_Gene: 5891
  • UniProtKB: Q9UQ07

Human MOK Predicted Functions

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in ciliary base. Predicted to be active in cytoplasm and nucleus. Biomarker of renal cell carcinoma.

Mouse Mok Target Information

Mouse Mok Predicted Functions

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to act upstream of or within phosphorylation. Located in ciliary base and nucleus. Is expressed in gonad. Orthologous to human MOK (MOK protein kinase).

Rat Mok Target Information

Rat Mok Predicted Functions

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in ciliary base. Predicted to be active in cytoplasm and nucleus. Orthologous to human MOK (MOK protein kinase).

MOK Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis

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