Human RAGE ELISA Kit

$245.00 – $350.00

Related Target	MOK
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.75 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	89% – 108%
Average Recovery	100%

ELISA Kit Detail Information

Related Target	MOK
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.75 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	89% – 108%
Average Recovery	100%
Intra Precision	3.4% – 5.0%
Inter Precision	5.2% – 5.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RAGE monoclonal antibody Human RAGE freeze-dried standard RAGE detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RAGE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RAGE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RAGE in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MOK

MOK Target Infomation Overview

Target Symbol: MOK, MOK protein kinase
Alias: RAGE1; STK30
Previous Names: RAGE
Alias Names: renal tumor antigen

MOK, MOK protein kinase Target Infomation by Species

Human
Mouse
Rat

Human MOK Target Information

Target Symbol: MOK, MOK protein kinase
Alias:
- MAPK/MAK/MRK overlapping kinase
- RAGE
- RAGE-1
- RAGE1
- renal cell carcinoma antigen
- renal cell carcinoma antigen (MOK protein kinase)
- renal tumor antigen 1
- STK30
NCBI_Gene: 5891
UniProtKB: Q9UQ07

Human MOK Predicted Functions

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in ciliary base. Predicted to be active in cytoplasm and nucleus. Biomarker of renal cell carcinoma.

Mouse Mok Target Information

Target Symbol: Mok, MOK protein kinase
Alias:
- MAPK/MAK/MRK/ overlapping kinase
- MOK
- Rage
- renal tumor antigen
- serine/threonine kinase 30
- Stk30
NCBI_Gene: 26448

Mouse Mok Predicted Functions

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in intracellular signal transduction. Predicted to act upstream of or within phosphorylation. Located in ciliary base and nucleus. Is expressed in gonad. Orthologous to human MOK (MOK protein kinase).

Rat Mok Target Information

Target Symbol: Mok, MOK protein kinase
Alias:
- LOC362787
- MAPK/MAK/MRK overlapping kinase
- MGC94988
- Rage
- renal tumor antigen
NCBI_Gene: 362787

Rat Mok Predicted Functions

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