Human RBP4 ELISA Kit

$245.00 – $350.00

Related Target	RBP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.05 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 110%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	RBP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.05 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 110%
Average Recovery	102%
Intra Precision	3.6% – 5.9%
Inter Precision	3.2% – 4.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RBP4 monoclonal antibody Human RBP4 freeze-dried standard RBP4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RBP4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RBP4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RBP4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

RBP4

RBP4 Target Infomation Overview

Target Symbol: RBP4, retinol binding protein 4
Gene Groups: Lipocalins
Alias Names: retinol-binding protein 4, plasma

RBP4, retinol binding protein 4 Target Infomation by Species

Human
Mouse
Rat

Human RBP4 Target Information

Target Symbol: RBP4, retinol binding protein 4
Alias:
- MCOPCB10
- plasma retinol-binding protein
- PRBP
- RBP
- RDCCAS
- retinol binding protein 4, plasma
- retinol-binding protein 4
- retinol-binding protein 4, interstitial
NCBI_Gene: 5950
UniProtKB: P02753

Human RBP4 Predicted Functions

Enables retinol binding activity. Involved in several processes, including gluconeogenesis; positive regulation of insulin secretion; and retinol metabolic process. Located in extracellular space. Implicated in type 2 diabetes mellitus. Biomarker of type 2 diabetes mellitus.

Mouse Rbp4 Target Information

Target Symbol: Rbp4, retinol binding protein 4, plasma
Alias:
- Rbp-4
- retinol binding protein 4, cellular
NCBI_Gene: 19662

Mouse Rbp4 Predicted Functions

Enables retinol binding activity. Involved in several processes, including animal organ development; gluconeogenesis; and negative regulation of cardiac muscle cell proliferation. Acts upstream of or within several processes, including detection of light stimulus involved in visual perception; retinoid metabolic process; and terpenoid transport. Located in extracellular space. Is expressed in several structures, including adipose tissue; brain; genitourinary system; gut; and hindlimb. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).

Rat Rbp4 Target Information

Target Symbol: Rbp4, retinol binding protein 4
Alias:
- plasma retinol-binding protein
- PRBP
- RBP
- RBPA
- retinol binding protein 4, plasma
- retinol-binding protein 4
NCBI_Gene: 25703
- UniprotKB: B2RZC1
- UniprotKB: P04916

Rat Rbp4 Predicted Functions

Enables retinol binding activity. Involved in response to ethanol and retinol transport. Located in extracellular space. Part of protein-containing complex. Biomarker of obesity and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).

Citations for Human RBP4 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.