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Human Resistin ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 2.67 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 108% |
| Average Recovery | 0.93 |
| Intra Precision | 4.6% – 5.9% |
| Inter-Precision | 5.0% – 6.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Resistin monoclonal antibody Human Resistin freeze-dried standard Resistin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Resistin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Resistin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Resistin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
RETN
RETN Target Infomation Overview
- Target Symbol: RETN, resistin
- Alias: FIZZ3; ADSF; RETN1
RETN, resistin Target Infomation by Species
HumanMouseRat
Human RETN Target Information
- Target Symbol: RETN, resistin
- Alias:
- adipose tissue-specific secretory factor
- ADSF
- C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
- c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
- cysteine-rich secreted protein A12-alpha-like 2
- cysteine-rich secreted protein FIZZ3
- FIZZ3
- found in inflammatory zone 3
- MGC126603
- MGC126609
- resistin delta2
- RETN1
- RSTN
- XCP1
- NCBI_Gene: 56729
- UniProtKB: Q9HD89
Human RETN Predicted Functions
Predicted to enable hormone activity. Predicted to be involved in several processes, including fat cell differentiation; negative regulation of feeding behavior; and positive regulation of cell communication. Located in extracellular exosome. Implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Biomarker of several diseases, including diabetes mellitus (multiple); diabetic neuropathy; heart disease (multiple); kidney failure (multiple); and systemic lupus erythematosus.
Mouse Retn Target Information
- Target Symbol: Retn, resistin
- Alias:
- adipocyte-specific secretory factor
- ADSF
- Fizz3
- rstn
- Xcp4
- NCBI_Gene: 57264
Mouse Retn Predicted Functions
Predicted to enable hormone activity. Predicted to be involved in several processes, including positive regulation of cell communication; positive regulation of smooth muscle cell migration; and positive regulation of smooth muscle cell proliferation. Located in extracellular space. Is expressed in adipose tissue; gonadal fat pad; inguinal fat pad; renal fat pad; and white fat. Human ortholog(s) of this gene implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Orthologous to human RETN (resistin).
Rat Retn Target Information
- Target Symbol: Retn, resistin
- Alias:
- 无
- NCBI_Gene: 246250
Rat Retn Predicted Functions
Predicted to enable hormone activity. Involved in several processes, including positive regulation of cell communication; positive regulation of smooth muscle cell migration; and positive regulation of smooth muscle cell proliferation. Located in extracellular space and nucleus. Used to study colitis. Biomarker of non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Orthologous to human RETN (resistin).
