Human Resistin ELISA Kit

$350.00$450.00

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TARGETSRETN
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.67 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 108%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.67 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 108%
Average Recovery0.93
Intra Precision4.6% – 5.9%
Inter-Precision5.0% – 6.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Resistin monoclonal antibody
Human Resistin freeze-dried standard
Resistin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Resistin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Resistin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Resistin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

RETN

RETN Target Infomation Overview

  • Target Symbol: RETN, resistin
  • Alias: FIZZ3; ADSF; RETN1

RETN, resistin Target Infomation by Species

HumanMouseRat

Human RETN Target Information

  • Target Symbol: RETN, resistin
  • Alias:
    • adipose tissue-specific secretory factor
    • ADSF
    • C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
    • c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
    • cysteine-rich secreted protein A12-alpha-like 2
    • cysteine-rich secreted protein FIZZ3
    • FIZZ3
    • found in inflammatory zone 3
    • MGC126603
    • MGC126609
    • resistin delta2
    • RETN1
    • RSTN
    • XCP1
  • NCBI_Gene: 56729
  • UniProtKB: Q9HD89

Human RETN Predicted Functions

Predicted to enable hormone activity. Predicted to be involved in several processes, including fat cell differentiation; negative regulation of feeding behavior; and positive regulation of cell communication. Located in extracellular exosome. Implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Biomarker of several diseases, including diabetes mellitus (multiple); diabetic neuropathy; heart disease (multiple); kidney failure (multiple); and systemic lupus erythematosus.

Mouse Retn Target Information

Mouse Retn Predicted Functions

Predicted to enable hormone activity. Predicted to be involved in several processes, including positive regulation of cell communication; positive regulation of smooth muscle cell migration; and positive regulation of smooth muscle cell proliferation. Located in extracellular space. Is expressed in adipose tissue; gonadal fat pad; inguinal fat pad; renal fat pad; and white fat. Human ortholog(s) of this gene implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Orthologous to human RETN (resistin).

Rat Retn Target Information

Rat Retn Predicted Functions

Predicted to enable hormone activity. Involved in several processes, including positive regulation of cell communication; positive regulation of smooth muscle cell migration; and positive regulation of smooth muscle cell proliferation. Located in extracellular space and nucleus. Used to study colitis. Biomarker of non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in hypertension; hypertrophic cardiomyopathy; obesity; and type 2 diabetes mellitus. Orthologous to human RETN (resistin).

RETN Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis

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