Human sVCAM-1/CD106 ELISA Kit

$350.00$450.00

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TARGETSVCAM1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(prediluted)
Sensitivity2.65 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 111%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(prediluted)
Sensitivity2.65 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 111%
Average Recovery0.99
Intra Precision1.9% – 2.6%
Inter-Precision3.2% – 5.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sVCAM-1/CD106 monoclonal antibody
Human sVCAM-1/CD106 freeze-dried standard
sVCAM-1/CD106 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sVCAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sVCAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sVCAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VCAM1

VCAM1 Target Infomation Overview

  • Target Symbol: VCAM1, vascular cell adhesion molecule 1
  • Gene Groups: CD molecules; Receptor ligands; C2-set domain containing; I-set domain containing; Ig-like cell adhesion molecule family
  • Alias: CD106

VCAM1, vascular cell adhesion molecule 1 Target Infomation by Species

HumanMouseRat

Human VCAM1 Target Information

  • Target Symbol: VCAM1, vascular cell adhesion molecule 1
  • Alias:
    • CD106
    • CD106 antigen
    • DKFZp779G2333
    • INCAM-100
    • MGC99561
    • vascular cell adhesion protein 1
  • NCBI_Gene: 7412
  • UniProtKB: P19320

Human VCAM1 Predicted Functions

Enables integrin binding activity and primary amine oxidase activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cell-cell adhesion in response to extracellular stimulus; and cellular response to amyloid-beta. Located in several cellular components, including early endosome; external side of plasma membrane; and podosome. Part of alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); essential thrombocythemia; glomerulonephritis (multiple); and interstitial cystitis.

Mouse Vcam1 Target Information

Mouse Vcam1 Predicted Functions

Predicted to enable integrin binding activity and primary amine oxidase activity. Acts upstream of or within several processes, including cellular response to glucose stimulus; chorio-allantoic fusion; and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Located in apical plasma membrane. Is expressed in several structures, including brain; embryo mesenchyme; extraembryonic component; heart; and hemolymphoid system. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human VCAM1 (vascular cell adhesion molecule 1).

Rat Vcam1 Target Information

Rat Vcam1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including cellular response to tumor necrosis factor; nervous system development; and response to lipopolysaccharide. Located in cell surface; extracellular space; and sarcolemma. Used to study carotid stenosis and hypertension. Biomarker of several diseases, including artery disease (multiple); chronic kidney disease; colitis; periodontitis; and thromboangiitis obliterans. Orthologous to human VCAM1 (vascular cell adhesion molecule 1).

VCAM1 Target News

    9
    ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury
    Cell Death & Disease
    4.004
    5-epi-ent-Kaurane diterpenoids from the aerial parts of Isodon eriocalyx and their anti-atherosclerotic potential
    PHYTOCHEMISTRY
    4.6
    Long-term abuse of caffeine sodium benzoate induces endothelial cells injury and leads to coagulation dysfunction
    IUBMB LIFE
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Systemic Lupus Erythematosus

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