Name: Human Thioredoxin-1/Trx-1 ELISA Kit
SKU: EK1222
Availability: InStock

EK1222

Human Thioredoxin-1/Trx-1 ELISA Kit

$245.00 – $350.00

Related Target	KMT2A
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	3.92 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	81% – 110%
Average Recovery	100%

ELISA Kit Detail Information

Related Target	KMT2A
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	3.92 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	81% – 110%
Average Recovery	100%
Intra Precision	3.9% – 4.9%
Inter Precision	4.9% – 5.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Thioredoxin-1/Trx-1 monoclonal antibody Human Thioredoxin-1/Trx-1 freeze-dried standard Thioredoxin-1/Trx-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Trx-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Trx-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Trx-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

KMT2A

KMT2A Target Infomation Overview

Target Symbol: KMT2A, lysine methyltransferase 2A
Gene Groups: PHD finger proteins; Zinc fingers CXXC-type; Lysine methyltransferases; Bromodomain containing; SET domain containing
Alias: TRX1; HRX; ALL-1; HTRX1; CXXC7; MLL1A; MLL1; ALL1; HTRX
Previous Names: MLL
Alias Names: Histone-lysine N-methyltransferase 2A; myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog); myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); lysine (K)-specific methyltransferase 2A; Trithorax-like protein; CXXC-type zinc finger protein 7; Acute lymphocytic 1

KMT2A, lysine methyltransferase 2A Target Infomation by Species

Human
Mouse
Rat

Human KMT2A Target Information

Target Symbol: KMT2A, lysine methyltransferase 2A
Alias:
- ALL-1
- CDK6/MLL fusion protein
- CXXC-type zinc finger protein 7
- CXXC7
- FLJ11783
- histone-lysine N-methyltransferase 2A
- histone-lysine N-methyltransferase MLL
- HRX
- HTRX1
- hypothetical protein FLJ11783
- lysine (K)-specific methyltransferase 2A
- lysine N-methyltransferase 2A
- mixed lineage leukemia 1
- MLL
- MLL-AF4 der(11) fusion protein
- MLL-AF9
- MLL/GAS7
- MLL/GAS7 fusion protein
- MLL/GMPS fusion protein
- MLL1
- MLL1A
- myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
- myeloid/lymphoid or mixed-lineage leukemia protein 1
- TET1-MLL
- trithorax-like protein
- TRX1
- WDSTS
- zinc finger protein HRX
NCBI_Gene: 4297
UniProtKB: Q03164

Human KMT2A Predicted Functions

Enables several functions, including lysine-acetylated histone binding activity; protein homodimerization activity; and protein methyltransferase activity. Involved in several processes, including histone modification; positive regulation of transporter activity; and regulation of nucleobase-containing compound metabolic process. Located in cytosol and nucleoplasm. Part of MLL1 complex. Implicated in acute myeloid leukemia; cervical cancer; mixed phenotype acute leukemia; and non-Hodgkin lymphoma. Biomarker of prostate cancer.

Mouse Kmt2a Target Information

Target Symbol: Kmt2a, lysine (K)-specific methyltransferase 2A
Alias:
- acute lymphocytic leukemia
- ALL-1
- All1
- Cxxc7
- HTRX1
- Mll
- Mll1
- myeloid/lymphoid or mixed-lineage leukemia
- myeloid/lymphoid or mixed-lineage leukemia 1
- trithorax Drosophila
NCBI_Gene: 214162

Mouse Kmt2a Predicted Functions

Enables DNA binding activity; chromatin binding activity; and histone methyltransferase activity (H3-K4 specific). Involved in several processes, including definitive hemopoiesis; regulation of gene expression; and regulation of histone modification. Acts upstream of or within several processes, including hematopoietic or lymphoid organ development; macromolecule methylation; and regulation of histone modification. Located in nucleus. Is expressed in several structures, including extraembryonic component; genitourinary system; hematopoietic system; nervous system; and retina. Used to study acute lymphoblastic leukemia and acute myeloid leukemia. Human ortholog(s) of this gene implicated in acute myeloid leukemia; cervical cancer; mixed phenotype acute leukemia; and non-Hodgkin lymphoma. Orthologous to human KMT2A (lysine methyltransferase 2A).

Rat Kmt2a Target Information

Target Symbol: Kmt2a, lysine methyltransferase 2A
Alias:
- histone-lysine N-methyltransferase 2A
- histone-lysine N-methyltransferase MLL
- lysine (K)-specific methyltransferase 2A
- Mixed-lineage leukemia (also acute lymphocytic leukemia 1 or trithorax Drosophila gene)
- Mixed-lineage leukemia (also acute lymphocytic leukemia 1 or tritorax Drosophila gene)
- Mll
- Mll_mapped
- Mll1
- myeloid/lymphoid or mixed-lineage leukemia
- myeloid/lymphoid or mixed-lineage leukemia (mapped)
- myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
- myeloid/lymphoid or mixed-lineage leukemia 1
NCBI_Gene: 315606
UniProtKB: F1M0L3

Rat Kmt2a Predicted Functions

Predicted to enable protein C-terminus binding activity. Involved in several processes, including DNA metabolic process; animal organ regeneration; and cellular response to heat. Located in nucleus. Used to study high grade glioma; leiomyoma; and prostate carcinoma. Biomarker of colon cancer; kidney disease; mucositis; type 2 diabetes mellitus; and urinary bladder cancer. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

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