Name: Human Thrombospondin-1/THBS-1 ELISA Kit
SKU: EK1155
Availability: InStock

EK1155

Human Thrombospondin-1/THBS-1 ELISA Kit

$245.00 – $350.00

Related Target	THBS1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	26.39 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	85% – 110%
Average Recovery	99%

ELISA Kit Detail Information

Related Target	THBS1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	26.39 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	85% – 110%
Average Recovery	99%
Intra Precision	2.3% – 2.5%
Inter Precision	3.5% – 5.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Thrombospondin-1/THBS-1 monoclonal antibody Human Thrombospondin-1/THBS-1 freeze-dried standard Thrombospondin-1/THBS-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human THBS-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, THBS-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of THBS-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

THBS1

THBS1 Target Infomation Overview

Target Symbol: THBS1, thrombospondin 1
Alias: TSP1; THBS; TSP; THBS-1; TSP-1
Alias Names: thrombospondin-1p180

THBS1, thrombospondin 1 Target Infomation by Species

Human
Mouse
Rat

Human THBS1 Target Information

Target Symbol: THBS1, thrombospondin 1
Alias:
- glycoprotein G
- THBS
- THBS-1
- thrombospondin-1
- thrombospondin-1p180
- thrombospondin-p50
- TSP
- TSP-1
- TSP1
NCBI_Gene: 7057
UniProtKB: P07996

Human THBS1 Predicted Functions

Enables several functions, including collagen V binding activity; fibrinogen binding activity; and fibroblast growth factor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of endothelial cell chemotaxis; negative regulation of endothelial cell proliferation; and negative regulation of nitric oxide mediated signal transduction. Located in several cellular components, including external side of plasma membrane; extracellular space; and platelet alpha granule. Part of fibrinogen complex. Colocalizes with collagen-containing extracellular matrix. Implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Biomarker of COVID-19; cholangiocarcinoma; obesity; pancreatic cancer (multiple); and urinary bladder cancer.

Mouse Thbs1 Target Information

Target Symbol: Thbs1, thrombospondin 1
Alias:
- tbsp1
- Thbs-1
- TSP-1
- TSP1
NCBI_Gene: 21825

Mouse Thbs1 Predicted Functions

Enables extracellular matrix binding activity. Involved in several processes, including behavioral response to pain; positive regulation of macrophage chemotaxis; and positive regulation of transforming growth factor beta receptor signaling pathway. Acts upstream of or within several processes, including cellular response to nitric oxide; circulatory system development; and monocyte aggregation. Located in extracellular space and sarcoplasmic reticulum. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and sensory organ. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).

Rat Thbs1 Target Information

Target Symbol: Thbs1, thrombospondin 1
Alias:
- thrombospondin-1
- TSP-1
- Tsp1
NCBI_Gene: 445442

Rat Thbs1 Predicted Functions

Enables transforming growth factor beta binding activity. Involved in several processes, including cellular response to tumor necrosis factor; positive regulation of transforming growth factor beta production; and response to testosterone. Located in cytoplasm and extracellular space. Biomarker of diabetic retinopathy; heart disease; myocardial infarction; and prostate cancer. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).

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