Human TIMP-2 ELISA Kit

$245.00 – $350.00

Related Target	TIMP2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.68 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 119%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	TIMP2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.68 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 119%
Average Recovery	102%
Intra Precision	3.8% – 4.1%
Inter Precision	4.2% - 4.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-2 monoclonal antibody Human TIMP-2 freeze-dried standard Human TIMP-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TIMP2

TIMP2 Target Infomation Overview

Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
Gene Groups: Tissue inhibitor of metallopeptidases
Alias: CSC-21K
Alias Names: tissue inhibitor of metalloproteinase 2

TIMP2, TIMP metallopeptidase inhibitor 2 Target Infomation by Species

Human
Mouse
Rat

Human TIMP2 Target Information

Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
Alias:
- CSC-21K
- DDC8
- KIAA1731NL
- metalloproteinase inhibitor 2
- testicular secretory protein Li 57
- TIMP-2
- tissue inhibitor of metalloproteinase 2
- tissue inhibitor of metalloproteinases 2
NCBI_Gene: 7077
UniProtKB: P16035

Human TIMP2 Predicted Functions

Enables peptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in negative regulation of metallopeptidase activity. Located in collagen-containing extracellular matrix. Implicated in Moyamoya disease. Biomarker of several diseases, including Duchenne muscular dystrophy; aortic disease (multiple); carcinoma (multiple); neurodegenerative disease (multiple); and preretinal fibrosis.

Mouse Timp2 Target Information

Target Symbol: Timp2, tissue inhibitor of metalloproteinase 2
Alias:
- D11Bwg1104e
- DNA segment, Chr 11, Brigham & Women’s Genetics 1104 expressed
- Timp-2
- TIMP-2
NCBI_Gene: 21858

Mouse Timp2 Predicted Functions

Enables enzyme activator activity; integrin binding activity; and metalloendopeptidase inhibitor activity. Acts upstream of or within several processes, including negative regulation of cell population proliferation; regulation of MAPK cascade; and regulation of neuron differentiation. Located in basement membrane and cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and skeletal system. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).

Rat Timp2 Target Information

Target Symbol: Timp2, TIMP metallopeptidase inhibitor 2
Alias:
- metalloproteinase inhibitor 2
- MGC105282
- TIMP-2
- tissue inhibitor of metalloproteinase 2
- tissue inhibitor of metalloproteinases 2
NCBI_Gene: 29543
UniProtKB: P30121

Rat Timp2 Predicted Functions

Enables integrin binding activity and metalloendopeptidase inhibitor activity. Involved in several processes, including positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; and regulation of intracellular signal transduction. Located in several cellular components, including cell surface; growth cone; and neuronal cell body. Used to study portal hypertension. Biomarker of cardiomyopathy; congestive heart failure; and intracranial aneurysm. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).

Citations for Human TIMP-2 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.