Name: Human TL-1A/TNFSF15 ELISA Kit
SKU: EK1268
Availability: InStock

EK1268

Human TL-1A/TNFSF15 ELISA Kit

$245.00 – $350.00

Related Target	TNFSF15
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	3.09 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	86% – 97%
Average Recovery	90%

ELISA Kit Detail Information

Related Target	TNFSF15
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	3.09 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	86% – 97%
Average Recovery	90%
Intra Precision	4.1% – 4.9%
Inter Precision	2.7% - 5.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TL-1A monoclonal antibody Human TL-1A freeze-dried standard Human TL-1A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TL-1A antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TL-1A present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TL-1A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TNFSF15

TNFSF15 Target Infomation Overview

Target Symbol: TNFSF15, TNF superfamily member 15
Gene Groups: Tumor necrosis factor superfamily
Alias: TL1; VEGI; TL1A; VEGI192A; MGC129934; MGC129935
Alias Names: vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial growth inhibitor-192A; tumor necrosis factor (ligand) superfamily, member 15

TNFSF15, TNF superfamily member 15 Target Infomation by Species

Human
Mouse
Rat

Human TNFSF15 Target Information

Target Symbol: TNFSF15, TNF superfamily member 15
Alias:
- MGC129934
- MGC129935
- TL1
- TL1A
- TNF ligand-related molecule 1
- TNF superfamily ligand TL1A
- TNLG1B
- tumor necrosis factor (ligand) superfamily member 15
- tumor necrosis factor (ligand) superfamily, member 15
- tumor necrosis factor ligand 1B
- tumor necrosis factor ligand superfamily member 15
- tumor necrosis factor superfamily member 15
- vascular endothelial cell growth inhibitor
- vascular endothelial growth inhibitor-192A
- VEGI
- VEGI192A
NCBI_Gene: 9966
UniProtKB: O95150

Human TNFSF15 Predicted Functions

Predicted to enable signaling receptor binding activity. Acts upstream of or within activation of NF-kappaB-inducing kinase activity and activation of cysteine-type endopeptidase activity involved in apoptotic process. Located in plasma membrane.

Mouse Tnfsf15 Target Information

Target Symbol: Tnfsf15, tumor necrosis factor (ligand) superfamily, member 15
Alias:
- TL1
- TL1A
- VEGI
NCBI_Gene: 326623

Mouse Tnfsf15 Predicted Functions

Predicted to enable death receptor binding activity. Predicted to be involved in activation of cysteine-type endopeptidase activity involved in apoptotic process and positive regulation of cytokine production. Predicted to act upstream of or within activation of NF-kappaB-inducing kinase activity. Predicted to be located in plasma membrane. Used to study inflammatory bowel disease 16. Orthologous to human TNFSF15 (TNF superfamily member 15).

Rat Tnfsf15 Target Information

Target Symbol: Tnfsf15, TNF superfamily member 15
Alias:
- TNF ligand-related molecule 1
- Tnlg1b
- tumor necrosis factor (ligand) superfamily, member 15
- tumor necrosis factor ligand 1b
- tumor necrosis factor ligand superfamily member 15
- tumor necrosis factor superfamily member 15
- vascular endothelial cell growth inhibitor
NCBI_Gene: 252878
- UniprotKB: A0A0H2UHG9
- UniprotKB: Q8K3Y7

Rat Tnfsf15 Predicted Functions

Enables death receptor binding activity. Involved in activation of cysteine-type endopeptidase activity involved in apoptotic process and positive regulation of cytokine production. Predicted to be located in plasma membrane. Orthologous to human TNFSF15 (TNF superfamily member 15).

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