Human Total HIF-1α/HIF1A ELISA Kit

$350.00$450.00

SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 1.55 pg/mL
Range 23.44 pg/mL – 1500 pg/mL
Assay Time 3.5 h
Recovery 83% – 123%
Average Recovery 1.00
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL (diluent)
Sensitivity 1.55 pg/mL
Range 23.44 pg/mL – 1500 pg/mL
Assay Time 3.5 h
Recovery 83% – 123%
Average Recovery 1.00
Intra Precision 5.3% – 7.6%
Inter-Precision 5.7% – 6.9%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human HIF-1αcapture antibody
Human HIF-1α freeze-dried standard
Human HIF-1α detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HIF-1α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HIF-1α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of HIF-1α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

6.7
Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α
INFLAMMATION RESEARCH

Related Products

more +

EK1341

$350.00$450.00

EK1C01

$350.00$450.00

EK1242

$350.00$450.00

EK1334

$350.00$450.00

EK2295

$350.00$450.00

EK1360

$350.00$450.00

EK2100

$350.00$450.00

EK224

$350.00$450.00

EK1349

$350.00$450.00

EK397

$350.00$450.00

EK285

$350.00$450.00

EK2153

$350.00$450.00

EK387

$350.00$450.00

EK2F01

$350.00$450.00

EK2313

$350.00$450.00

EK2382

$350.00$450.00

Same Target