Human Total HIF-1α/HIF1A ELISA Kit

$350.00$450.00

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SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity1.55 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery83% – 123%
Average Recovery1.00
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity1.55 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery83% – 123%
Average Recovery1.00
Intra Precision5.3% – 7.6%
Inter-Precision5.7% – 6.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human HIF-1αcapture antibody
Human HIF-1α freeze-dried standard
Human HIF-1α detect Antibody
Streptavidin-HRP
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HIF-1α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HIF-1α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of HIF-1α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

6.986
Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α
INFLAMMATION RESEARCH

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