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Human Total HIF-1α/HIF1A ELISA Kit
$350.00 – $450.00
| SPECIES | Human |
|---|---|
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 1.55 pg/mL |
| Range | 23.44 pg/mL – 1500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 83% – 123% |
| Average Recovery | 1.00 |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 1.55 pg/mL |
| Range | 23.44 pg/mL – 1500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 83% – 123% |
| Average Recovery | 1.00 |
| Intra Precision | 5.3% – 7.6% |
| Inter-Precision | 5.7% – 6.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human HIF-1αcapture antibody Human HIF-1α freeze-dried standard Human HIF-1α detect Antibody Streptavidin-HRP Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HIF-1α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HIF-1α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of HIF-1α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
