Name: Mouse Total HIF-1α/HIF1A ELISA Kit
SKU: EK2392
Availability: InStock

EK2392

Mouse Total HIF-1α/HIF1A ELISA Kit

$299.00 – $419.00

DATASHEET

Related Target	hif1a
Species	mouse
Sample Type	Cell lysate
Sample Volume	Cell lysate：10 μL
Sensitivity	0.46 pg/mL
Array Range	23.44 pg/mL - 1500 pg/mL
Assay Time	3.5 h
Recovery	81% - 117%
Average Recovery	97%

SKU: EK2392 Category: ELISA Kit

ELISA Kit Detail Information

Related Target	hif1a
Species	mouse
Sample Type	Cell lysate
Sample Volume	Cell lysate：10 μL
Sensitivity	0.46 pg/mL
Array Range	23.44 pg/mL - 1500 pg/mL
Assay Time	3.5 h
Recovery	81% - 117%
Average Recovery	97%
Intra Precision	4.5% - 6.3%
Inter Precision	7.1% - 7.5%
Plate	Detachable 96-well Plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ Blue Ice Transport
Components	1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Detect Antibody Standard Diluent Streptavidin-HRP Standard Microplate Wells
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human HIF-1α/HIF1A has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for HIF-1α/HIF1A are pipetted into the wells and HIF-1α/HIF1A present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of HIF-1α/HIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured

Related Targets

hif1a

No description available.

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