EK2392

Mouse Total HIF-1α/HIF1A ELISA Kit

$245.00$350.00

Related Target
Speciesmouse
Sample TypeCell lysate
Sample VolumeCell lysate:10 μL
Sensitivity0.46 pg/mL
Array Range23.44 pg/mL - 1500 pg/mL
Assay Time3.5 h
Recovery81% - 117%
Average Recovery97%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeCell lysate
Sample VolumeCell lysate:10 μL
Sensitivity0.46 pg/mL
Array Range23.44 pg/mL - 1500 pg/mL
Assay Time3.5 h
Recovery81% - 117%
Average Recovery97%
Intra Precision4.5% - 6.3%
Inter Precision7.1% - 7.5%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Detect Antibody
Standard Diluent
Streptavidin-HRP
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human HIF-1α/HIF1A has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for HIF-1α/HIF1A are pipetted into the wells and HIF-1α/HIF1A present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of HIF-1α/HIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured

Related Targets

hif1a

No description available.

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