Human VEGF-C ELISA Kit

$245.00 – $350.00

Related Target	VEGFC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	4.24 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 119%
Average Recovery	96%

ELISA Kit Detail Information

Related Target	VEGFC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	4.24 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 119%
Average Recovery	96%
Intra Precision	3.6% – 7.0%
Inter Precision	3.3% – 6.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF-C monoclonal antibody Human VEGF-C freeze-dried standard VEGF-C detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF-C antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF-C present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of VEGF-C in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

VEGFC

VEGFC Target Infomation Overview

Target Symbol: VEGFC, vascular endothelial growth factor C
Gene Groups: VEGF family
Alias: VRP; VEGF-C
Alias Names: vascular endothelial growth factor-related protein

VEGFC, vascular endothelial growth factor C Target Infomation by Species

Human
Mouse
Rat

Human VEGFC Target Information

Target Symbol: VEGFC, vascular endothelial growth factor C
Alias:
- FLT4 ligand DHM
- Flt4-L
- LMPH1D
- LMPHM4
- vascular endothelial growth factor-related protein
- VEGF-C
- VRP
NCBI_Gene: 7424
UniProtKB: P49767

Human VEGFC Predicted Functions

Enables chemoattractant activity and vascular endothelial growth factor receptor 3 binding activity. Involved in induction of positive chemotaxis; positive regulation of mast cell chemotaxis; and vascular endothelial growth factor receptor signaling pathway. Predicted to be located in extracellular region and platelet alpha granule lumen. Predicted to be active in extracellular space. Implicated in breast carcinoma and hereditary lymphedema ID. Biomarker of several diseases, including invasive ductal carcinoma; liver disease (multiple); papillary carcinoma; pterygium; and urinary system cancer (multiple).

Mouse Vegfc Target Information

Target Symbol: Vegfc, vascular endothelial growth factor C
Alias:
- AW228853
- expressed sequence AW228853
- VEGF-C
NCBI_Gene: 22341
- UniprotKB: P97953

Mouse Vegfc Predicted Functions

Enables vascular endothelial growth factor receptor 3 binding activity. Involved in several processes, including negative regulation of osteoblast differentiation; positive regulation of angiogenesis; and positive regulation of mesenchymal stem cell proliferation. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; positive regulation of lymphangiogenesis; and positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in several structures, including alimentary system mesentery; brain; cardiovascular system; embryo mesenchyme; and genitourinary system. Used to study hereditary lymphedema. Human ortholog(s) of this gene implicated in breast carcinoma and hereditary lymphedema ID. Orthologous to human VEGFC (vascular endothelial growth factor C).

Rat Vegfc Target Information

Target Symbol: Vegfc, vascular endothelial growth factor C
Alias:
- flt4 ligand
- flt4-L
- vascular endothelial growth factor-related protein
- VEGF-C
- VRP
NCBI_Gene: 114111
UniProtKB: O35757

Rat Vegfc Predicted Functions

Enables vascular endothelial growth factor receptor 3 binding activity. Involved in several processes, including positive regulation of blood vessel endothelial cell migration; positive regulation of protein autophosphorylation; and regulation of vascular endothelial growth factor receptor signaling pathway. Acts upstream of or within positive regulation of neuroblast proliferation. Predicted to be located in membrane. Predicted to be active in extracellular space. Biomarker of colon adenocarcinoma. Human ortholog(s) of this gene implicated in breast carcinoma and hereditary lymphedema ID. Orthologous to human VEGFC (vascular endothelial growth factor C).

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