Human VEGF ELISA Kit

$350.00$450.00

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TARGETSVEGFA
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.95 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery77% – 119%
Average Recovery1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.95 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery77% – 119%
Average Recovery1
Intra Precision3.3% – 4.5%
Inter-Precision4.2% – 6.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF monoclonal antibody
Human VEGF freeze-dried standard
VEGF detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VEGFA

VEGFA Target Infomation Overview

  • Target Symbol: VEGFA, vascular endothelial growth factor A
  • Gene Groups: VEGF family
  • Alias: VEGF-A; VPF
  • Previous Names: VEGF
  • Alias Names: vascular endothelial growth factor

VEGFA, vascular endothelial growth factor A Target Infomation by Species

HumanMouseRat

Human VEGFA Target Information

  • Target Symbol: VEGFA, vascular endothelial growth factor A
  • Alias:
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • vascular permeability factor
    • VEGF
    • VEGF-A
    • VPF
  • NCBI_Gene: 7422
  • UniProtKB: P15692

Human VEGFA Predicted Functions

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

Mouse Vegfa Target Information

Mouse Vegfa Predicted Functions

Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

Rat Vegfa Target Information

Rat Vegfa Predicted Functions

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

VEGFA Target News

    5.8
    Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis
    Cancer Cell International
    2.9
    Dihydroartemisinin inhibits angiogenesis in breast cancer via regulating VEGF and MMP-2/-9
    FUNDAMENTAL & CLINICAL PHARMACOLOGY
    3.6
    Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis
    AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
    10.9
    Enhanced antibacterial activity, corrosion resistance and endothelialization potential of Ti-5Cu alloy by oxygen and nitrogen plasma-based surface modification
    JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
    12.822
    Duo-role Platelet-rich Plasma: Temperature-induced Fibrin Gel and Growth Factors’ Reservoir for Microneedles to Promote Hair Regrowth
    Journal of Advanced Research
    5.714
    P-Hydroxylcinnamaldehyde induces tumor-associated macrophage polarization toward the M1 type by regulating the proteome and inhibits ESCC in vivo and in vitro
    INTERNATIONAL IMMUNOPHARMACOLOGY
    8.025
    3D-bioprinted double-crosslinked angiogenic alginate/chondroitin sulfate patch for diabetic wound healing
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    5.8
    Immunomodulatory effect of Ti-Cu alloy by surface nanostructure synergistic with Cu2+ release
    COLLOIDS AND SURFACES B-BIOINTERFACES
    4.85
    Exosomal B7-H3 facilitates colorectal cancer angiogenesis and metastasis through AKT1/mTOR/VEGFA pathway
    CELLULAR SIGNALLING
    4.374
    FOXN3 inhibits the progression of ovarian cancer through negatively regulating the expression of RPS15A
    Human Cell
    2.6
    Upregulation of SEMP1 Contributes to Improving the Biological Functions of Trophoblast via the PI3K/AKT Pathway in Preeclampsia
    MOLECULAR BIOTECHNOLOGY
    2.6
    EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal
    MOLECULAR BIOTECHNOLOGY
    6.986
    Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α
    INFLAMMATION RESEARCH
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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