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Human VEGF R1/Flt-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 6.99 pg/mL |
| Range | 0.16 ng/mL – 10 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 96% – 114% |
| Average Recovery | 0.99 |
| Intra Precision | 4.6% – 5.5% |
| Inter-Precision | 2.9% – 4.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF R1/Flt-1 monoclonal antibody Human VEGF R1/Flt-1 freeze-dried standard VEGF R1/Flt-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF R1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF R1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF R1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
FLT1
FLT1 Target Infomation Overview
- Target Symbol: FLT1, fms related receptor tyrosine kinase 1
- Gene Groups: Receptor tyrosine kinases; I-set domain containing
- Alias: VEGFR1
- Previous Names: FLT
- Alias Names: vascular endothelial growth factor receptor 1; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); fms-related tyrosine kinase 1; fms related tyrosine kinase 1
FLT1, fms related receptor tyrosine kinase 1 Target Infomation by Species
HumanMouseRat
Human FLT1 Target Information
- Target Symbol: FLT1, fms related receptor tyrosine kinase 1
- Alias:
- FLT
- FLT-1
- fms related tyrosine kinase 1
- fms-like tyrosine kinase 1
- fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
- tyrosine-protein kinase FRT
- tyrosine-protein kinase receptor FLT
- vascular endothelial growth factor receptor 1
- vascular permeability factor receptor
- VEGFR-1
- VEGFR1
- NCBI_Gene: 2321
- UniProtKB: P17948
Human FLT1 Predicted Functions
Enables growth factor binding activity; placental growth factor-activated receptor activity; and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including monocyte chemotaxis; positive regulation of kinase activity; and protein phosphorylation. Located in actin cytoskeleton and plasma membrane. Is integral component of plasma membrane. Part of receptor complex. Implicated in Kuhnt-Junius degeneration; diabetic retinopathy; and pre-eclampsia. Biomarker of several diseases, including Kawasaki disease; Leydig cell tumor; cerebrovascular disease (multiple); kidney cancer (multiple); and retinal disease (multiple).
Mouse Flt1 Target Information
- Target Symbol: Flt1, FMS-like tyrosine kinase 1
- Alias:
- AI323757
- expressed sequence AI323757
- Flt-1
- sFlt1
- vascular endothelial growth factor receptor-1
- VEGFR-1
- VEGFR1
- NCBI_Gene: 14254
Mouse Flt1 Predicted Functions
Enables identical protein binding activity and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including blood vessel morphogenesis; embryonic morphogenesis; and hyaloid vascular plexus regression. Acts upstream of or within several processes, including angiogenesis; positive regulation of hematopoietic progenitor cell differentiation; and post-embryonic camera-type eye morphogenesis. Located in extracellular space and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in Kuhnt-Junius degeneration; diabetic retinopathy; and pre-eclampsia. Orthologous to human FLT1 (fms related receptor tyrosine kinase 1).
Rat Flt1 Target Information
- Target Symbol: Flt1, Fms related receptor tyrosine kinase 1
- Alias:
- FLT-1
- FMS-like tyrosine kinase 1
- FMS-related tyrosine kinase 1
- Fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
- LOC100911280
- tyrosine-protein kinase receptor FLT
- vascular endothelial growth factor receptor 1
- vascular endothelial growth factor receptor 1-like
- vascular endothelial growth factor/vascular permeability factor receptor
- VEGFR-1
- NCBI_Gene: 54251
Rat Flt1 Predicted Functions
Enables ATP binding activity; growth factor binding activity; and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including liver regeneration; negative regulation of vascular endothelial growth factor production; and positive regulation of cell population proliferation. Located in extracellular space and plasma membrane. Used to study corneal neovascularization. Biomarker of breast cancer; diabetic retinopathy; liver cirrhosis; myocardial infarction; and vascular dementia. Human ortholog(s) of this gene implicated in Kuhnt-Junius degeneration; diabetic retinopathy; and pre-eclampsia. Orthologous to human FLT1 (fms related receptor tyrosine kinase 1).
