Human VEGF R2/KDR ELISA Kit

$350.00$450.00

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TARGETSKDR
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.52 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery88% – 107%
Average Recovery0.94
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.52 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery88% – 107%
Average Recovery0.94
Intra Precision3.7% – 4.0%
Inter-Precision3.6% – 3.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF R2/KDR monoclonal antibody
Human VEGF R2/KDR freeze-dried standard
VEGF R2/KDR detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF R2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF R2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF R2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

KDR

KDR Target Infomation Overview

  • Target Symbol: KDR, kinase insert domain receptor
  • Gene Groups: Receptor tyrosine kinases; CD molecules; V-set domain containing; I-set domain containing
  • Alias: FLK1; VEGFR; VEGFR2; CD309
  • Alias Names: vascular endothelial growth factor receptor 2; fetal liver kinase 1; kinase insert domain receptor (a type III receptor tyrosine kinase)

KDR, kinase insert domain receptor Target Infomation by Species

HumanMouseRat

Human KDR Target Information

  • Target Symbol: KDR, kinase insert domain receptor
  • Alias:
    • CD309
    • fetal liver kinase 1
    • fetal liver kinase-1
    • FLK1
    • kinase insert domain receptor (a type III receptor tyrosine kinase)
    • protein-tyrosine kinase receptor Flk-1
    • soluble VEGFR2
    • tyrosine kinase growth factor receptor
    • vascular endothelial growth factor receptor 2
    • VEGFR
    • VEGFR2
  • NCBI_Gene: 3791
  • UniProtKB: P35968

Human KDR Predicted Functions

Enables several functions, including cell adhesion molecule binding activity; vascular endothelial growth factor binding activity; and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including intracellular signal transduction; positive regulation of endothelial cell migration; and protein phosphorylation. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of mitochondrial depolarization; and positive regulation of mitochondrial fission. Located in several cellular components, including Golgi apparatus; endosome; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); gastrointestinal system cancer (multiple); glioblastoma; macular degeneration (multiple); and pancreatic cancer (multiple). Biomarker of several diseases, including breast cancer (multiple); carcinoma (multiple); cerebrovascular disease (multiple); eye disease (multiple); and systemic scleroderma (multiple).

Mouse Kdr Target Information

Mouse Kdr Predicted Functions

Enables growth factor binding activity and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including circulatory system development; peptidyl-tyrosine autophosphorylation; and positive regulation of vasculogenesis. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in several cellular components, including cell junction; endosome; and external side of plasma membrane. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in several diseases, including adhesions of uterus; artery disease (multiple); gastrointestinal system cancer (multiple); macular degeneration (multiple); and pancreatic cancer (multiple). Orthologous to human KDR (kinase insert domain receptor).

Rat Kdr Target Information

  • Target Symbol: Kdr, kinase insert domain receptor
  • Alias:
    • fetal liver kinase 1
    • FLK-1
    • FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2)
    • FLK1 kinase insert domain receptor (VEGF receptor 2)
    • kinase insert domain protein receptor
    • MGC93590
    • protein-tyrosine kinase receptor flk-1
    • vascular endothelial growth factor receptor 2
    • Vegfr-2
  • NCBI_Gene: 25589

Rat Kdr Predicted Functions

Enables growth factor binding activity and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including generation of neurons; positive regulation of endothelial cell proliferation; and positive regulation of intracellular signal transduction. Located in several cellular components, including neuron projection; neuronal cell body; and plasma membrane. Used to study adhesions of uterus; brain ischemia; cystitis; and myocardial infarction. Biomarker of several diseases, including artery disease (multiple); impotence; ovarian hyperstimulation syndrome; prostate disease (multiple); and retinopathy of prematurity. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); gastrointestinal system cancer (multiple); glioblastoma; macular degeneration (multiple); and pancreatic cancer (multiple). Orthologous to human KDR (kinase insert domain receptor).

KDR Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)

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