Name: Human VEGF R3/Flt-4 ELISA Kit
SKU: EK1197
Availability: InStock

EK1197

Human VEGF R3/Flt-4 ELISA Kit

$245.00 – $350.00

Related Target	FLT4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	15.35 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	79% – 110%
Average Recovery	95%

ELISA Kit Detail Information

Related Target	FLT4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	15.35 pg/mL
Array Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	79% – 110%
Average Recovery	95%
Intra Precision	4.3% – 9.0%
Inter Precision	7.2% – 10.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF R3/Flt-4 monoclonal antibody Human VEGF R3/Flt-4 freeze-dried standard VEGF R3/Flt-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF R3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, VEGF R3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF R3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

FLT4

FLT4 Target Infomation Overview

Target Symbol: FLT4, fms related receptor tyrosine kinase 4
Gene Groups: Receptor tyrosine kinases; I-set domain containing
Alias: VEGFR3; PCL; VEGFR-3
Alias Names: vascular endothelial growth factor receptor 3; VEGF receptor-3; Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4; primary congenital lymphedema; fms-related tyrosine kinase 4; fms related tyrosine kinase 4

FLT4, fms related receptor tyrosine kinase 4 Target Infomation by Species

Human
Mouse
Rat

Human FLT4 Target Information

Target Symbol: FLT4, fms related receptor tyrosine kinase 4
Alias:
- CHTD7
- Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4
- FLT-4
- FLT41
- fms related tyrosine kinase 4
- fms-like tyrosine kinase 4
- fms-related tyrosine kinase 4
- LMPH1A
- LMPHM1
- PCL
- primary congenital lymphedema
- soluble VEGFR3 variant 1
- soluble VEGFR3 variant 2
- soluble VEGFR3 variant 3
- tyrosine-protein kinase receptor FLT4
- vascular endothelial growth factor receptor 3
- VEGF receptor-3
- VEGFR-3
- VEGFR3
NCBI_Gene: 2324
UniProtKB: P35916

Human FLT4 Predicted Functions

Enables several functions, including protein homodimerization activity; protein phosphatase binding activity; and vascular endothelial growth factor-activated receptor activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and protein phosphorylation. Acts upstream of or within vascular endothelial growth factor receptor signaling pathway. Located in cytosol; nucleoplasm; and plasma membrane. Part of receptor complex. Implicated in hereditary lymphedema IA. Biomarker of lymphangioma and systemic scleroderma.

Mouse Flt4 Target Information

Target Symbol: Flt4, FMS-like tyrosine kinase 4
Alias:
- AI323512
- Chy
- chylous ascites
- expressed sequence AI323512
- Flt-4
- VEGFR-3
- VEGFR3
NCBI_Gene: 14257
- UniprotKB: P35917
- UniprotKB: Q5SU94

Mouse Flt4 Predicted Functions

Enables vascular endothelial growth factor-activated receptor activity. Involved in lymphangiogenesis; regulation of blood vessel remodeling; and sprouting angiogenesis. Acts upstream of or within lung alveolus development; positive regulation of cell population proliferation; and respiratory system process. Predicted to be located in cytosol; nucleoplasm; and plasma membrane. Predicted to be part of receptor complex. Predicted to be integral component of plasma membrane. Is expressed in several structures, including alimentary system; branchial arch; cardiovascular system; central nervous system; and extraembryonic component. Used to study hereditary lymphedema. Human ortholog(s) of this gene implicated in hereditary lymphedema IA. Orthologous to human FLT4 (fms related receptor tyrosine kinase 4).

Rat Flt4 Target Information

Target Symbol: Flt4, Fms related receptor tyrosine kinase 4
Alias:
- FLT-4
- FMS-like tyrosine kinase 4
- fms-related tyrosine kinase 4
- LOC360235
- tyrosine-protein kinase receptor FLT4
- vascular endothelial growth factor receptor 3
- VEGFR-3
- Vegfr3
NCBI_Gene: 114110
- UniprotKB: G3V6B7
- UniprotKB: Q91ZT1

Rat Flt4 Predicted Functions

Enables growth factor binding activity and vascular endothelial growth factor-activated receptor activity. Involved in positive regulation of cell growth and vascular endothelial growth factor receptor signaling pathway. Predicted to be located in cytosol; nucleoplasm; and plasma membrane. Predicted to be part of receptor complex. Predicted to be integral component of plasma membrane. Human ortholog(s) of this gene implicated in hereditary lymphedema IA. Orthologous to human FLT4 (fms related receptor tyrosine kinase 4).

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