Mouse B7-H1/PD-L1/CD274 ELISA Kit

$350.00$450.00

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TARGETSCD274
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Range31.3 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery83% – 108%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Range31.3 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery83% – 108%
Average Recovery0.93
Intra Precision1.9% – 3.2%
Inter-Precision4.0%-4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PD-L1 monoclonal antibody
Mouse PD-L1 freeze-dried standard
PD-L1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse PD-L1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PD-L1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PD-L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD274

CD274 Target Infomation Overview

  • Target Symbol: CD274, CD274 molecule
  • Gene Groups: CD molecules; Receptor ligands; V-set domain containing; C2-set domain containing; B7 family
  • Alias: B7-H; B7H1; PD-L1; PDL1; B7-H1
  • Previous Names: PDCD1LG1
  • Alias Names: B7 homolog 1; programmed cell death 1 ligand 1; CD274 antigen

CD274, CD274 molecule Target Infomation by Species

HumanMouseRat

Human CD274 Target Information

  • Target Symbol: CD274, CD274 molecule
  • Alias:
    • B7 homolog 1
    • B7-H
    • B7H1
    • CD274 antigen
    • hPD-L1
    • MGC142294
    • MGC142296
    • PD-L1
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL1
    • programmed cell death 1 ligand 1
    • programmed death ligand 1
  • NCBI_Gene: 29126
  • UniProtKB: Q9NZQ7

Human CD274 Predicted Functions

Involved in several processes, including positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process; regulation of T cell activation; and regulation of cytokine production. Located in several cellular components, including endosome membrane; extracellular exosome; and nucleoplasm. Biomarker of several diseases, including Cryptococcal meningitis; Human papillomavirus infectious disease; gastrointestinal system cancer (multiple); lymphoma (multiple); and tuberculosis (multiple).

Mouse Cd274 Target Information

Mouse Cd274 Predicted Functions

Acts upstream of or within negative regulation of T cell proliferation and toxin transport. Located in external side of plasma membrane. Is expressed in retina and thymus primordium. Orthologous to human CD274 (CD274 molecule).

Rat Cd274 Target Information

  • Target Symbol: Cd274, CD274 molecule
  • Alias:
    • CD274 antigen
    • LOC499342
    • Pdcd1lg1
    • programmed cell death 1 ligand 1
    • RGD1566211
    • similar to PD-1-ligand precursor
  • NCBI_Gene: 499342
  • UniProtKB: D4AE25

Rat Cd274 Predicted Functions

Involved in positive regulation of T cell proliferation and positive regulation of cell migration. Located in cell surface. Biomarker of periodontitis and pre-eclampsia. Orthologous to human CD274 (CD274 molecule).

CD274 Target News

    5.714
    Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway
    INTERNATIONAL IMMUNOPHARMACOLOGY
    Multiple Sclerosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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