Mouse CXCL16 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL16
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.09 pg/mL
Range	1.25 pg/mL – 80 pg/mL
Assay Time	3.5 h
Recovery	93% – 115%
Average Recovery	1.03

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.09 pg/mL
Range	1.25 pg/mL – 80 pg/mL
Assay Time	3.5 h
Recovery	93% – 115%
Average Recovery	1.03
Intra Precision	2.7% – 4.3%
Inter-Precision	3.0%-4.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL16 monoclonal antibody Mouse CXCL16 freeze-dried standard CXCL16 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CXCL16 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL16 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL16 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CXCL16

CXCL16 Target Infomation Overview

Target Symbol: CXCL16, C-X-C motif chemokine ligand 16
Gene Groups: Chemokine ligands; Scavenger receptors
Alias: SR-PSOX; CXCLG16; SRPSOX
Alias Names: CXC chemokine ligand 16; chemokine (C-X-C motif) ligand 16

CXCL16, C-X-C motif chemokine ligand 16 Target Infomation by Species

HumanMouseRat

Human CXCL16 Target Information

Target Symbol: CXCL16, C-X-C motif chemokine ligand 16
Alias:
- C-X-C motif chemokine 16
- chemokine (C-X-C motif) ligand 16
- CXC chemokine ligand 16
- CXCLG16
- scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
- small-inducible cytokine B16
- SR-PSOX
- SRPSOX
- transmembrane chemokine CXCL16
NCBI_Gene: 58191
UniProtKB: Q9H2A7

Human CXCL16 Predicted Functions

Enables chemokine activity. Involved in several processes, including positive regulation of cell growth; response to interferon-gamma; and response to tumor necrosis factor. Located in extracellular space. Biomarker of COVID-19 and systemic scleroderma.

Mouse Cxcl16 Target Information

Target Symbol: Cxcl16, chemokine (C-X-C motif) ligand 16
Alias:
- 0910001K24Rik
- AV290116
- BB024863
- expressed sequence AV290116
- expressed sequence BB024863
- RIKEN cDNA 0910001K24 gene
- Scavenger Receptor
- SR-PSOX
- SR-PSOX/CXCL16
NCBI_Gene: 66102

Mouse Cxcl16 Predicted Functions

Enables chemokine activity; low-density lipoprotein particle receptor activity; and scavenger receptor activity. Involved in T cell chemotaxis and cellular response to lipopolysaccharide. Located in extracellular space. Is integral component of membrane. Is expressed in bladder; female associated reproductive structure; hindbrain meninges; male associated reproductive structure; and spinal cord meninges. Orthologous to human CXCL16 (C-X-C motif chemokine ligand 16).

Rat Cxcl16 Target Information

Target Symbol: Cxcl16, C-X-C motif chemokine ligand 16
Alias:
- C-X-C motif chemokine 16
- chemokine (C-X-C motif) ligand 16
- similar to chemokine (C-X-C motif) ligand 16
- small-inducible cytokine B16
- transmembrane chemokine CXCL16
NCBI_Gene: 497942

Rat Cxcl16 Predicted Functions

Predicted to enable chemokine activity; low-density lipoprotein particle receptor activity; and scavenger receptor activity. Predicted to be involved in several processes, including T cell chemotaxis; cellular response to lipopolysaccharide; and response to interferon-gamma. Predicted to be integral component of membrane. Predicted to be active in extracellular space. Orthologous to human CXCL16 (C-X-C motif chemokine ligand 16).