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Mouse gp130 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 1.44 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 112% |
| Average Recovery | 1.02 |
| Intra Precision | 2.6% – 3.5% |
| Inter-Precision | 2.5%-4.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-gp130 monoclonal antibody Mouse gp130 freeze-dried standard Mouse gp130 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse gp130 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, gp130 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of gp130 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
LRPPRC
LRPPRC Target Infomation Overview
- Target Symbol: LRPPRC, leucine rich pentatricopeptide repeat containing
- Gene Groups: Mitochondrial respiratory chain complex assembly factors; Pentatricopeptide repeat containing
- Alias: GP130; LRP130
- Previous Names: LSFC
- Alias Names: Leigh syndrome, French-Canadian type (cytochrome oxidase deficiency)
LRPPRC, leucine rich pentatricopeptide repeat containing Target Infomation by Species
HumanMouseRat
Human LRPPRC Target Information
- Target Symbol: LRPPRC, leucine rich pentatricopeptide repeat containing
- Alias:
- 130 kDa leucine-rich protein
- CLONE-23970
- GP130
- leucine-rich pentatricopeptide repeat containing
- leucine-rich PPR motif-containing protein, mitochondrial
- leucine-rich PPR-motif containing
- LRP 130
- LRP130
- LSFC
- MC4DN5
- mitochondrial leucine-rich PPR motif-containing protein
- NCBI_Gene: 10128
- UniProtKB: P42704
Human LRPPRC Predicted Functions
Enables beta-tubulin binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in mitochondrion transport along microtubule. Predicted to act upstream of or within negative regulation of mitochondrial RNA catabolic process and regulation of mitochondrial translation. Located in several cellular components, including condensed nuclear chromosome; mitochondrial nucleoid; and perinuclear region of cytoplasm. Colocalizes with mitochondrion. Implicated in French Canadian Leigh disease and Leigh disease.
Mouse Lrpprc Target Information
- Target Symbol: Lrpprc, leucine-rich PPR-motif containing
- Alias:
- 3110001K13Rik
- C76645
- expressed sequence C76645
- Lrp130
- RIKEN cDNA 3110001K13 gene
- NCBI_Gene: 72416
Mouse Lrpprc Predicted Functions
Enables RNA binding activity and single-stranded DNA binding activity. Acts upstream of or within negative regulation of mitochondrial RNA catabolic process and regulation of mitochondrial translation. Located in mitochondrion and nucleus. Part of ribonucleoprotein complex. Is expressed in several structures, including brain; genitourinary system; heart; liver; and skeletal muscle. Human ortholog(s) of this gene implicated in French Canadian Leigh disease and Leigh disease. Orthologous to human LRPPRC (leucine rich pentatricopeptide repeat containing).
Rat Lrpprc Target Information
- Target Symbol: Lrpprc, leucine-rich pentatricopeptide repeat containing
- Alias:
- 130 kDa leucine-rich protein
- leucine rich protein 157
- leucine-rich PPR motif-containing protein, mitochondrial
- leucine-rich PPR-motif containing
- leucine-rich protein 157
- LOC313867
- LRP 130
- Lrp157
- rLRP157
- NCBI_Gene: 313867
Rat Lrpprc Predicted Functions
Predicted to enable beta-tubulin binding activity; nucleic acid binding activity; and ubiquitin protein ligase binding activity. Predicted to be involved in mRNA transport. Predicted to act upstream of or within negative regulation of mitochondrial RNA catabolic process and regulation of mitochondrial translation. Predicted to be located in several cellular components, including condensed nuclear chromosome; mitochondrial nucleoid; and perinuclear region of cytoplasm. Predicted to be part of ribonucleoprotein complex. Predicted to colocalize with mitochondrion. Human ortholog(s) of this gene implicated in French Canadian Leigh disease and Leigh disease. Orthologous to human LRPPRC (leucine rich pentatricopeptide repeat containing).
