Mouse IL-17AF ELISA Kit

$299.00 – $419.00

DATASHEET

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	4.54 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	73% – 125%
Average Recovery	110%

SKU: EK2177 Category: ELISA Kit

ELISA Kit Detail Information

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	4.54 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	73% – 125%
Average Recovery	110%
Intra Precision	4.4% – 7.7%
Inter Precision	4.7% – 6.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17AF monoclonal antibody Mouse IL-17AF freeze-dried standard IL-17AF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-17AF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17AF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17AF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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