Mouse IL-1ra/IL-1F3 ELISA Kit

$350.00$450.00

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TARGETSIL1RN
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.70 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 109%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.70 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 109%
Average Recovery0.93
Intra Precision3.5% – 4.3%
Inter-Precision4.5%-4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1ra monoclonal antibody
Mouse IL-1ra freeze-dried standard
Mouse IL-1ra detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1ra antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1ra present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1ra in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1RN

IL1RN Target Infomation Overview

  • Target Symbol: IL1RN, interleukin 1 receptor antagonist
  • Gene Groups: Interleukins; Interleukin receptors
  • Alias: IL1RA; ICIL-1RA; IL1F3; IRAP; IL-1RN; MGC10430
  • Alias Names: interleukin-1 receptor antagonist protein; intracellular interleukin-1 receptor antagonist

IL1RN, interleukin 1 receptor antagonist Target Infomation by Species

HumanMouseRat

Human IL1RN Target Information

  • Target Symbol: IL1RN, interleukin 1 receptor antagonist
  • Alias:
    • DIRA
    • ICIL-1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RN (IL1F3)
    • interleukin-1 receptor antagonist protein
    • intracellular IL-1 receptor antagonist type II
    • intracellular interleukin-1 receptor antagonist (icIL-1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • type II interleukin-1 receptor antagonist
  • NCBI_Gene: 3557
  • UniProtKB: P18510

Human IL1RN Predicted Functions

Enables cytokine receptor binding activity. Involved in several processes, including acute-phase response; negative regulation of heterotypic cell-cell adhesion; and negative regulation of interleukin-1-mediated signaling pathway. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); chronic kidney disease (multiple); glomerulonephritis (multiple); lung disease (multiple); and obesity (multiple).

Mouse Il1rn Target Information

Mouse Il1rn Predicted Functions

Predicted to enable cytokine receptor binding activity. Acts upstream of or within insulin secretion and lipid metabolic process. Located in extracellular region and vesicle. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).

Rat Il1rn Target Information

Rat Il1rn Predicted Functions

Enables interleukin-1 receptor binding activity. Involved in several processes, including cellular response to norepinephrine stimulus; chronic inflammatory response to antigenic stimulus; and fever generation. Located in extracellular space. Used to study adult respiratory distress syndrome; anti-basement membrane glomerulonephritis; hypertension; silicosis; and transient cerebral ischemia. Biomarker of encephalitis and pneumonia. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).

IL1RN Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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