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Mouse IL-1ra/IL-1F3 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 0.70 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 88% – 109% |
| Average Recovery | 0.93 |
| Intra Precision | 3.5% – 4.3% |
| Inter-Precision | 4.5%-4.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1ra monoclonal antibody Mouse IL-1ra freeze-dried standard Mouse IL-1ra detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1ra antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1ra present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1ra in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL1RN
IL1RN Target Infomation Overview
- Target Symbol: IL1RN, interleukin 1 receptor antagonist
- Gene Groups: Interleukins; Interleukin receptors
- Alias: IL1RA; ICIL-1RA; IL1F3; IRAP; IL-1RN; MGC10430
- Alias Names: interleukin-1 receptor antagonist protein; intracellular interleukin-1 receptor antagonist
IL1RN, interleukin 1 receptor antagonist Target Infomation by Species
HumanMouseRat
Human IL1RN Target Information
- Target Symbol: IL1RN, interleukin 1 receptor antagonist
- Alias:
- DIRA
- ICIL-1RA
- IL-1ra
- IL-1ra3
- IL-1RN
- IL1 inhibitor
- IL1F3
- IL1RA
- IL1RN (IL1F3)
- interleukin-1 receptor antagonist protein
- intracellular IL-1 receptor antagonist type II
- intracellular interleukin-1 receptor antagonist (icIL-1ra)
- IRAP
- MGC10430
- MVCD4
- type II interleukin-1 receptor antagonist
- NCBI_Gene: 3557
- UniProtKB: P18510
Human IL1RN Predicted Functions
Enables cytokine receptor binding activity. Involved in several processes, including acute-phase response; negative regulation of heterotypic cell-cell adhesion; and negative regulation of interleukin-1-mediated signaling pathway. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); chronic kidney disease (multiple); glomerulonephritis (multiple); lung disease (multiple); and obesity (multiple).
Mouse Il1rn Target Information
- Target Symbol: Il1rn, interleukin 1 receptor antagonist
- Alias:
- F630041P17Rik
- IL-1ra
- RIKEN cDNA F630041P17 gene
- NCBI_Gene: 16181
Mouse Il1rn Predicted Functions
Predicted to enable cytokine receptor binding activity. Acts upstream of or within insulin secretion and lipid metabolic process. Located in extracellular region and vesicle. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).
Rat Il1rn Target Information
- Target Symbol: Il1rn, interleukin 1 receptor antagonist
- Alias:
- IL-1ra
- IL-1RN
- IL1 inhibitor
- interleukin 1 receptor antagonist gene
- interleukin-1 receptor antagonist protein
- IRAP
- NCBI_Gene: 60582
Rat Il1rn Predicted Functions
Enables interleukin-1 receptor binding activity. Involved in several processes, including cellular response to norepinephrine stimulus; chronic inflammatory response to antigenic stimulus; and fever generation. Located in extracellular space. Used to study adult respiratory distress syndrome; anti-basement membrane glomerulonephritis; hypertension; silicosis; and transient cerebral ischemia. Biomarker of encephalitis and pneumonia. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).
