Mouse IL-1ra/IL-1F3 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL1RN
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.70 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 109%
Average Recovery	0.93

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.70 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 109%
Average Recovery	0.93
Intra Precision	3.5% – 4.3%
Inter-Precision	4.5%-4.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1ra monoclonal antibody Mouse IL-1ra freeze-dried standard Mouse IL-1ra detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1ra antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1ra present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1ra in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL1RN

IL1RN Target Infomation Overview

Target Symbol: IL1RN, interleukin 1 receptor antagonist
Gene Groups: Interleukins; Interleukin receptors
Alias: IL1RA; ICIL-1RA; IL1F3; IRAP; IL-1RN; MGC10430
Alias Names: interleukin-1 receptor antagonist protein; intracellular interleukin-1 receptor antagonist

IL1RN, interleukin 1 receptor antagonist Target Infomation by Species

HumanMouseRat

Human IL1RN Target Information

Target Symbol: IL1RN, interleukin 1 receptor antagonist
Alias:
- DIRA
- ICIL-1RA
- IL-1ra
- IL-1ra3
- IL-1RN
- IL1 inhibitor
- IL1F3
- IL1RA
- IL1RN (IL1F3)
- interleukin-1 receptor antagonist protein
- intracellular IL-1 receptor antagonist type II
- intracellular interleukin-1 receptor antagonist (icIL-1ra)
- IRAP
- MGC10430
- MVCD4
- type II interleukin-1 receptor antagonist
NCBI_Gene: 3557
UniProtKB: P18510

Human IL1RN Predicted Functions

Enables cytokine receptor binding activity. Involved in several processes, including acute-phase response; negative regulation of heterotypic cell-cell adhesion; and negative regulation of interleukin-1-mediated signaling pathway. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); chronic kidney disease (multiple); glomerulonephritis (multiple); lung disease (multiple); and obesity (multiple).

Mouse Il1rn Target Information

Target Symbol: Il1rn, interleukin 1 receptor antagonist
Alias:
- F630041P17Rik
- IL-1ra
- RIKEN cDNA F630041P17 gene
NCBI_Gene: 16181

Mouse Il1rn Predicted Functions

Predicted to enable cytokine receptor binding activity. Acts upstream of or within insulin secretion and lipid metabolic process. Located in extracellular region and vesicle. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).

Rat Il1rn Target Information

Target Symbol: Il1rn, interleukin 1 receptor antagonist
Alias:
- IL-1ra
- IL-1RN
- IL1 inhibitor
- interleukin 1 receptor antagonist gene
- interleukin-1 receptor antagonist protein
- IRAP
NCBI_Gene: 60582

Rat Il1rn Predicted Functions

Enables interleukin-1 receptor binding activity. Involved in several processes, including cellular response to norepinephrine stimulus; chronic inflammatory response to antigenic stimulus; and fever generation. Located in extracellular space. Used to study adult respiratory distress syndrome; anti-basement membrane glomerulonephritis; hypertension; silicosis; and transient cerebral ischemia. Biomarker of encephalitis and pneumonia. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).