Mouse IL-2 ELISA Kit

$350.00$450.00

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TARGETSIL2
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.54 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery93% – 113%
Average Recovery1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.54 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery93% – 113%
Average Recovery1
Intra Precision3.3% – 6.0%
Inter-Precision5.1% – 9.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- IL-2 monoclonal antibody
Mouse IL-2 freeze-dried standard
IL-2 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL2

IL2 Target Infomation Overview

  • Target Symbol: IL2, interleukin 2
  • Gene Groups: Interleukins
  • Alias: IL-2; TCGF
  • Alias Names: T cell growth factor

IL2, interleukin 2 Target Infomation by Species

HumanMouseRat

Human IL2 Target Information

  • Target Symbol: IL2, interleukin 2
  • Alias:
    • aldesleukin
    • IL-2
    • interleukin-2
    • involved in regulation of T-cell clonal expansion
    • lymphokine
    • T cell growth factor
    • TCGF
  • NCBI_Gene: 3558
  • UniProtKB: P60568

Human IL2 Predicted Functions

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).

Mouse Il2 Target Information

Mouse Il2 Predicted Functions

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren’s syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

Rat Il2 Target Information

Rat Il2 Predicted Functions

Enables carbohydrate binding activity; glycosphingolipid binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of T cell activation; protein kinase C-activating G protein-coupled receptor signaling pathway; and regulation of protein phosphorylation. Located in extracellular space. Used to study cystitis. Biomarker of allergic rhinitis; colorectal carcinoma; and tongue squamous cell carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

IL2 Target News

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    Nutrients
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    Synergistic Antitumor Effect of Grifola frondose Polysaccharide—Protein Complex in Combination with Cyclophosphamide in H22 Tumor-Bearing Mice
    MOLECULES
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    Blueberry–Mulberry Extract Alleviates Cognitive Impairment, Regulates Gut Metabolites, and Inhibits Inflammation in Aged Mice
    Foods
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    Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain
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    7.4
    Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer
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    3
    Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo
    Chemical Biology & Drug Design
    17.1
    A Vanadium-Based Nanoplatform Synergizing Ferroptotic-like Therapy with Glucose Metabolism Intervention for Enhanced Cancer Cell Death and Antitumor Immunity
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    BIOMEDICINE & PHARMACOTHERAPY
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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