Mouse IL-6 ELISA Kit

$350.00$450.00

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TARGETSIL6
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.56 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery89% – 112%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.56 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery89% – 112%
Average Recovery1.02
Intra Precision2.3% – 3.0%
Inter-Precision2.1% – 5.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Mouse IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL6

IL6 Target Infomation Overview

  • Target Symbol: IL6, interleukin 6
  • Gene Groups: Interferons; Interleukins; Interleukin 6 type cytokine family
  • Alias: IL-6; BSF2; HGF; HSF
  • Previous Names: IFNB2
  • Alias Names: interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6, interleukin 6 Target Infomation by Species

HumanMouseRat

Human IL6 Target Information

  • Target Symbol: IL6, interleukin 6
  • Alias:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • NCBI_Gene: 3569
  • UniProtKB: P05231

Human IL6 Predicted Functions

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

Mouse Il6 Target Information

Mouse Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

Rat Il6 Target Information

  • Target Symbol: Il6, interleukin 6
  • Alias:
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • NCBI_Gene: 24498
  • UniProtKB: P20607

Rat Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

IL6 Target News

    3.7
    Involvement of estrogen receptor activation in kaempferol-3-O-glucoside’s protection against aging-related cognition impairment and microglial inflammation
    EXPERIMENTAL CELL RESEARCH
    3.3
    GPR34 knockdown relieves cognitive deficits and suppresses neuroinflammation in Alzheimer’s disease via the ERK/NF-κB signal
    NEUROSCIENCE
    9.417
    Engineered inhalable nanocatalytic therapeutics for Parkinson’s disease by inducing mitochondrial autophagy
    MATERIALS & DESIGN
    4
    Repositioning of clinically approved drug Bazi Bushen capsule for treatment of Aizheimer’s disease using network pharmacology approach and in vitro experimental validation
    Heliyon
    4
    Network pharmacology and in vivo studies reveal the neuroprotective effects of paeoniflorin on Alzheimer’s disease
    Heliyon
    6.7
    Design, synthesis and biological evaluation of carbamate derivatives incorporating multifunctional carrier scaffolds as pseudo-irreversible cholinesterase inhibitors for the treatment of Alzheimer’s disease
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
    6.2
    Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson’s disease: targeting neuroinflammation
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    7.7
    GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferases
    eLife
    3.6
    Pen Yan Jing Tablets Alleviates Pelvic Inflammatory Disease by Inhibiting Akt/NF-κB Pathway
    International Journal of Medical Sciences
    Telotristat Etiprate alleviates rheumatoid arthritis by targeting LGALS3 and affecting MAPK signaling
    6.058
    Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos
    Neural Regeneration Research
    3.4
    TIFA contributes to periodontitis in diabetic mice via activating the NF‑κB signaling pathway
    Molecular Medicine Reports
    2.7
    Microascus cirrosus SZ 2021: A potentially new genotype of Microascus cirrosus, which can cause fatal pulmonary infection in patients with acute leukemia following haplo‑HSCT
    Experimental and Therapeutic Medicine
    0.664
    Ginsenoside Rd Attenuates Lung Ischemia/Reperfusion Injury in Mice via Regulating Mitochondrial Function
    INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
    5.4
    Enhancing the Treatment of Uncontrolled Inflammation through the Targeted Delivery of TPCA-1-Loaded Nanoparticles
    Pharmaceutics
    4.967
    Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis and Gut Flora in Mice
    Polymers
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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